| Autor: |
Keith L. Ligon, Rameen Beroukhim, Pratiti Bandopadhayay, Robert Bachoo, Ralf Kittler, Sanda Alexandrescu, Susan Chi, Brian M. Alexander, Patrick Y. Wen, Pascale Varlet, Melanie Pages, Adrian Dubuc, Azra H. Ligon, Frederik De Smet, Alain Charest, Stanley Chaleff, Benjamin Carcamo, Rishi Lulla, Arnault Tauziede-Espariat, David Reardon, Katie Fehnel, Mark Kieran, Karen Wright, Liliana Goumnerova, Veerle Haemels, Michael Y. Tolstorukov, Matthew A. Booker, Elizabeth F. Cohen, Claire Sinai, Fiona Watkinson, Sarah Becker, Kristine Pelton, Jayne Vogelzang, Robert Jones, Dayle Wang, Jessica Tsai, Kathleen Schoolcraft, Frank Dubois, Lori Ramkissoon, Shakti Ramkissoon, Jared K. Woods, Seth Malinowski, Guadalupe Garcia, Maya Srikanth Graham, Ross Giglio, Anne-Florence Blandin |
| Rok vydání: |
2023 |
| Popis: |
Supplementary Figure 6: Single cell RNA sequencing comparison of ALK expression and other infant/pediatric glioma kinase drivers in developing human and mouse brain. A. 2D representation of human brain cell subtypes (48 samples) during cortex development using a single cell RNA-seq atlas (http://cells.ucsc.edu/?ds=cortex-dev) shows that ALK positive cells make up less than 1% of all cells in the dataset and aren't localized within a specific cluster.B. Cluster-level summary of the human scRNA dataset shows that ALK expression (TPM) is decreased compared to EGFR, NTRK2, and NTRK3, and ALK positive cells make up a much lower percentage of each cluster compared to those 3 genes.C. 2D representation of developmental mouse forebrain (E12.5 – P6) in a single cell RNA-seq atlas (http://cc-shiny-01.functionalgenomics.ca/braindex/clusters) shows similar results as seen in the human scRNA Nowakowski dataset.D. Cluster-level summary of the mouse scRNA dataset corroborates results seen in the Nowkowski scRNA dataset; that Alk expression (log-normalized) is decreased, and less prevalent than EGFR, NTRK2, and NTRK3.E. 2D representation of RNA sequencing data for NTRK1-3 genes in the Nowakowski developing human cortex dataset. Compared to ALK expression, the NTRK genes have increased expression levels, increased prevalence in the dataset, and show increased localization to specific cluster.F. Similar to (E), the 2D representation of Ntrk genes in the developing mouse scRNA dataset shows increased expression and prevalence compared to Alk. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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