A simple method for culture and stable maintenance of giant spheroplasts from the yeast Saccharomyces cerevisiae

Autor:	Tomohiro Kotani, Isamu Miyakawa, Taihei Fukudomi
Rok vydání:	2011
Předmět:	Lysis Ascomycota Cell division fungi Saccharomyces cerevisiae Mycology Spheroplasts Cell concentration Deoxyglucose Biology Spheroplast biology.organism_classification Applied Microbiology and Biotechnology Microbiology Yeast Culture Media Cell biology Saccharomyces Nuclear division Cell Division
Zdroj:	The Journal of General and Applied Microbiology. 57:177-182
ISSN:	1349-8037 0022-1260
DOI:	10.2323/jgam.57.177
Popis:	When spheroplasts of the yeast Saccharomyces cerevisiae are cultured in liquid medium containing osmotic stabilizer, they undergo nuclear division and growth without cell division, resulting in the formation of giant spheroplasts with multinuclei. In this study, we report a simple method for the culture and stable maintenance of giant spheroplasts. The selection of culture media and cell concentration was found to be important for the growth and maintenance of giant spheroplasts. Among the conditions that we tested, static culture in a synthetic Burkholder's medium in 96-well U-bottomed culture plates was most effective. Under appropriate conditions, we could maintain giant spheroplasts for more than 6 days without proliferation of whole cells or marked lysis. The average diameter of spheroplasts can vary from 16 to 53µm, depending on their initial concentration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::052c912d3e2e5fded7f7e33784aaf7fb https://doi.org/10.2323/jgam.57.177 Zobrazit plný text záznamu