Protein cross-linking, peroxidase and b-1,3-endoglucanase involved in resistance of pea against Orobanche crenata
| Autor: | Miguel A. Dita, Diego Rubiales, M. Dolores Lozano, C. I. González-Verdejo, Pablo González-Melendi, Alejandro Pérez-de-Luque, María Carmen Risueño, Jose Ignacio Cubero |
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| Rok vydání: | 2006 |
| Předmět: |
Physiology
Parasitic plant glucanase Fluorescent Antibody Technique peroxidase Plant Science Orobanche crenata Plant Roots Cell wall resistance chemistry.chemical_compound protein cross-linking Botany Cellulases Pisum sativum Microscopy Confocal biology Orobanche Host (biology) Glucan Endo-1 3-beta-D-Glucosidase Callose Peas biology.organism_classification Confocal microscopy chemistry biology.protein Cytochemistry parasitic plants in situ hybridization Central cylinder Peroxidase |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2005-0178 |
| Popis: | 9 páginas, 5 figuras -- PAGS nros. 1461-1469 Root holoparasitic angiosperms, like Orobanche spp, completely lack chlorophyll and totally depend on their host for their supply of nutrients. O. crenata is a severe constraint to the cultivation of legumes and breeding for resistance remains the most economical, feasible, and environmentally friendly method of control. Due to the lack of resistance in commercial pea cultivars, the use of wild relatives for breeding is necessary, and an understanding of the mechanisms underlying host resistance is needed in order to improve screening for resistance in breeding programmes. Compatible and incompatible interactions between O. crenata and pea have been studied using cytochemical procedures. The parasite was stopped in the host cortex before reaching the central cylinder, and accumulation of H2O2, peroxidases, and callose were detected in neighbouring cells. Protein cross-linking in the host cell walls appears as the mechanism of defence, halting penetration of the parasite. In situ hybridization studies have also shown that a peroxidase and a β-glucanase are differently expressed in cells of the resistant host (Pf651) near the penetration point. The role of these proteins in the resistance to O. crenata is discussed This research was supported by the projects AGL2005-01781, Food-CT2004-50622, and BOS2002-03550 |
| Databáze: | OpenAIRE |
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