lncRNA ADAMTS9-AS2 Controls Human Mesenchymal Stem Cell Chondrogenic Differentiation and Functions as a ceRNA
| Autor: | Yi-Fu Zhuang, Mingjian Huang, Jingyu Zhao, Jiajia Xu, Jing Li, Xiaoling Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
endocrine system cartilage defect Cellular differentiation Biology Article 03 medical and health sciences 0302 clinical medicine chondrogenesis Drug Discovery Gene expression chondrogenic differentiation cartilage regeneration Transcription factor MiR-942-5p Competing endogenous RNA lcsh:RM1-950 Mesenchymal stem cell Chondrogenesis Antisense RNA Cell biology LncRNA ADAMTS9-AS2 lcsh:Therapeutics. Pharmacology 030104 developmental biology 030220 oncology & carcinogenesis Molecular Medicine hMSCs Transforming growth factor |
| Zdroj: | Molecular Therapy: Nucleic Acids, Vol 18, Iss, Pp 533-545 (2019) Molecular Therapy. Nucleic Acids |
| ISSN: | 2162-2531 |
| DOI: | 10.1016/j.omtn.2019.08.027 |
| Popis: | Long noncoding RNAs (lncRNAs) have emerged as key regulators of cell differentiation and development. However, potential roles for lncRNAs in chondrogenic differentiation have remained poorly understood. Here we identify lncRNA ADAMTS9 antisense RNA 2, ADAMTS9-AS2, which controls the chondrogenic differentiation by acting as a competing endogenous RNA (ceRNA) in human mesenchymal stem cells (hMSCs). We screen out ADAMTS9-AS2 of undifferentiated and differentiated cells during chondrogenic differentiation by microarrays. Suppression or overexpression of lncRNA ADAMTS9-AS2 correlates with inhibition and promotion of hMSC chondrogenic differentiation, respectively. We find that ADAMTS9-AS2 can sponge miR-942-5p to regulate the expression of Scrg1, a transcription factor promoting chondrogenic gene expression. Finally, we confirm the function of ADAMTS9-AS2 to cartilage repair in the absence of transforming growth factor β (TGF-β) in vivo. In conclusion, ADAMTS9-AS2 plays an important role in chondrogenic differentiation as a ceRNA, so that it can be regarded as a therapy target for cartilage repair. Keywords: LncRNA ADAMTS9-AS2, MiR-942-5p, chondrogenesis, hMSCs, cartilage regeneration, chondrogenic differentiation, cartilage defect |
| Databáze: | OpenAIRE |
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