Population replacement of benzimidazole-resistant Haemonchus contortus with susceptible strains: evidence of changes in the resistance status

Autor: Sebastián Muchiut, César Fiel, Juan Pedro Lirón, Mercedes Lloberas, Carolina Ceriani, Ramiro Lorenzo, Eliana Riva, Gisele Bernat, Patricia Cardozo, Silvina Fernández, Pedro Steffan
Rok vydání: 2022
Předmět:
Zdroj: Parasitology Research. 121:2623-2632
ISSN: 1432-1955
0932-0113
DOI: 10.1007/s00436-022-07582-9
Popis: The spread of anthelmintic resistance (AR) in nematode populations threatens the viability of sheep production systems worldwide, and warrants the adoption of sensitive, practical, and standardized tests to detect AR. The aim of this study was to characterize the replacement of an Haemonchus contortus population resistant to benzimidazoles (BZDs) by a susceptible one, by means of both phenotypic and genotypic techniques. Phenotypic methods to assess BZD resistance included in vivo tests, such as the fecal egg count reduction test (FECRT), and in vitro tests, such as the egg hatch assay (EHA). Additionally, genotypification of polymorphisms associated with BZD resistance by sequencing a fragment of the isotype 1 β-tubulin gene was carried out. The initial, BZD-resistant population (initial Balcarce population) exhibited an egg count reduction (ECR) of 59.3%. Following refugium replacement, the final population (final Balcarce population) exhibited an ECR of 95.2%. For the initial Balcarce population, the median effective dose (ED
Databáze: OpenAIRE
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