C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1
| Autor: | Torsten Witte, Christèle Combes, Anne Kathrin Wessig, Annika Klingberg, Konstantin Neumann, Korbinian Brand, Andreas Pich, Anika Alberts |
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| Přispěvatelé: | Hannover Medical School [Hannover] (MHH), Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Hannover Medical School (GERMANY), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Centre Interuniversitaire de Recherche et d'Ingénierie des Matériaux - CIRIMAT (Toulouse, France) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Proteases Gout Matériaux Médecine humaine et pathologie lcsh:Medicine Plasma protein binding [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endopeptidases medicine Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology lcsh:Science C-reactive protein (CRP) Innate immunity Multidisciplinary biology Chemistry lcsh:R C-reactive protein Pattern recognition receptor Complement C3 medicine.disease Body Fluids Uric Acid 3. Good health Uric acid crystals C-Reactive Protein 030104 developmental biology Biochemistry [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system Mannose-Binding Protein-Associated Serine Proteases biology.protein Uric acid Female lcsh:Q Crystallization MASP1 Protein Binding Crystal deposition arthropathies 030215 immunology |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2020, 10, pp.6391. ⟨10.1038/s41598-020-63318-8⟩ Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-63318-8⟩ |
| Popis: | Gout is caused by crystallization of uric acid in the form of monosodium urate (MSU) crystals, which induce a sterile inflammatory response that is hardly distinguishable from microbe-induced inflammatory responses. It is unclear, if MSU crystals (like microbes) are recognized by specific pattern recognition receptors. To identify possible soluble pattern recognition molecules for MSU crystals, we purified MSU-binding proteins from human body fluids. We identified C-reactive protein (CRP) as a major MSU-binding protein. Binding of CRP was strong enough to specifically deplete CRP from human serum. We found that CRP was required for fixation of complement components C1q, C1r, C1s and MASP1. Thus, we have identified a pattern recognition molecule for MSU crystals that links to the activation of complement. Notably, CRP does not show an even binding to the complete surface of the crystals. It rather binds to edges or distinct faces of the crystals. |
| Databáze: | OpenAIRE |
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