The Cf-4 and Cf-9 Resistance Genes Against Cladosporium fulvum are Conserved in Wild Tomato Species

Autor: M. Kruijt, B.F. Brandwagt, Diana J Kip, Pierre J. G. M. de Wit, Matthieu H. A. J. Joosten
Rok vydání: 2005
Předmět:
Agroinfiltration
hypersensitive response
DNA
Plant
balancing selection
Physiology
Molecular Sequence Data
plant-disease-resistance
arabidopsis-thaliana
Plant disease resistance
Genes
Plant
genus lycopersicon
Lycopersicon
Conserved sequence
Evolution
Molecular
Open Reading Frames
Solanum lycopersicum
Species Specificity
Sequence Homology
Nucleic Acid
Botany
Arabidopsis thaliana
locus comprises
Wild tomato
Amino Acid Sequence
Selection
Genetic
race-specific elicitor
Conserved Sequence
Phylogeny
Plant Diseases
Plant Proteins
Genetics
Base Sequence
Sequence Homology
Amino Acid
biology
EPS-2
avirulence protein avr4
General Medicine
biology.organism_classification
leucine-rich repeat
Laboratorium voor Phytopathologie
Elicitor
Laboratory of Phytopathology
recognition
Cladosporium
Agronomy and Crop Science
Zdroj: Molecular Plant-Microbe Interactions, 18(9), 1011-1021
Molecular Plant-Microbe Interactions 18 (2005) 9
ISSN: 1943-7706
0894-0282
DOI: 10.1094/mpmi-18-1011
Popis: The Cf-4 and Cf-9 genes originate from the wild tomato species Lycopersicon hirsutum and L. pimpinellifolium and confer resistance to strains of the leaf mold fungus Cladosporium fulvum that secrete the Avr4 and Avr9 elicitor proteins, respectively. Homologs of Cf-4 and Cf-9 (Hcr9s) are located in several clusters and evolve mainly through sequence exchange between homologs. To study the evolution of Cf genes, we set out to identify functional Hcr9s that mediate recognition of Avr4 and Avr9 (designated Hcr9-Avr4s and Hcr9-Avr9s) in all wild tomato species. Plants responsive to the Avr4 and Avr9 elicitor proteins were identified throughout the genus Lycopersicon. Open reading frames of Hcr9s from Avr4- and Avr9-responsive tomato plants were polymerase chain reaction-amplified. Several Hcr9s that mediate Avr4 or Avr9 recognition were identified in diverged tomato species by agroinfiltration assays. These Hcr9-Avr4s and Hcr9-Avr9s are highly identical to Cf-4 and Cf-9, respectively. Therefore, we conclude that both Cf-4 and Cf-9 predate Lycopersicon speciation. These results further suggest that C. fulvum is an ancient pathogen of the genus Lycopersicon, in which Cf-4 and Cf-9 have been maintained by selection pressure imposed by C. fulvum.
Databáze: OpenAIRE
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