A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
| Autor: | Tomohiko Ishigami, Masatake Asano, Daisuke Omagari, Satsuki Saito, Miho Obayashi-Ishii |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_treatment
Blotting Western Enzyme-Linked Immunosorbent Assay In Vitro Techniques Real-Time Polymerase Chain Reaction law.invention 03 medical and health sciences 0302 clinical medicine law Biomarkers Tumor Tumor Cells Cultured medicine Humans Secretion Luciferase RNA Messenger General Dentistry Polymerase chain reaction Platelet-Derived Growth Factor Messenger RNA Chemistry NF-kappa B 030206 dentistry equipment and supplies Magnetostatics Molecular biology Blot Magnetic Fields Platelet-Derived Growth Factor-AA Cytokine 030220 oncology & carcinogenesis Carcinoma Squamous Cell Cytokines Mouth Neoplasms human activities |
| Zdroj: | Journal of Oral Science. 60:374-380 |
| ISSN: | 1880-4926 1343-4934 |
| DOI: | 10.2334/josnusd.17-0380 |
| Popis: | Magnetic attachments are commonly used for overdentures. The deleterious effects of exposure to magnetic flux on human health have not been substantiated so far; nevertheless, there is a need to understand the extent of magnetic field exposure in the oral area resulting from the use of magnetic attachments. The purpose of this study was to investigate the influence of a magnetic field on oral squamous cell carcinoma. Tumor cells cultured on a magnetic plate were compared with those not cultured on a magnetic plate (controls). The cells were seeded at a density of 1 × 105 cells/well and cultured for 6 days. The influence of the magnetic field on cytokine production was examined by cytokine array analysis. Secretion of platelet-derived growth factor-AA (PDGF-AA) was measured by enzyme-linked immunosorbent assay and Western blotting. The expression of PDGF-AA messenger RNA was examined by real-time polymerase chain reaction, whereas nuclear factor-kappa B activity was measured by luciferase assay. The results indicated that the magnetic field inhibited the secretion of PDGF-AA, thereby inhibiting PDGF-AA-induced expression, thus reducing the risk of cancer recurrence. |
| Databáze: | OpenAIRE |
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