Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5
| Autor: | Jose Flower Valor, Geert Angenon, Willy Dillen, Janniek De Clercq, Alain Goossens, Riet De Rycke, C. Cardona, Marc Van Montagu, Constanza Quintero |
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| Přispěvatelé: | Vrije Universiteit Brussel, Plant Genetics |
| Rok vydání: | 2000 |
| Předmět: |
Phaseolus
chemistry.chemical_classification Mexican bean weevil Physiology Zabrotes subfasciatus food and beverages Alpha amylase inhibitor Locus (genetics) Plant Science Genetically modified crops Biology arcelin biology.organism_classification chemistry Genetic marker Botany Storage protein Cultivar bruchid resistance Gene |
| Zdroj: | Journal of Experimental Botany. 51:1229-1236 |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Arcelins are abundant seed storage proteins thought to be implicated in the resistance of wild Phaseolus vulgaris (L.) genotypes against Zabrotes subfasciatus (Boheman), an important storage insect pest of common bean. Here, the insecticidal activity of the arcelin‐5 variant that is present in the highly resistant P. vulgaris accession G02771 was investigated. No correlation could be established between the presence of arcelin 5 and the insecticidal effects observed in G02771 seeds. Insect feeding assays with artificial seeds into which purified arcelin‐5 protein was incorporated and with transgenic P. acutifolius (A. Gray) seeds in which the arcelin‐5 genes were expressed, showed that the presence of arcelin‐5 proteins, even at elevated levels, was not sufficient to achieve adequate resistance against Z. subfasciatus. The same might apply to other arcelin variants. Nevertheless, as resistance is clearly closely linked to the presence of the arcelin‐1 or arcelin‐5 locus, arcelins remain useful markers in breeding programmes aimed at introgressing high levels of resistance to Z. subfasciatus in P. vulgaris cultivars. |
| Databáze: | OpenAIRE |
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