RCH51, a multiply antibiotic-resistant Acinetobacter baumannii ST103IP isolate, carries resistance genes in three plasmids, including a novel potentially conjugative plasmid carrying oxa235 in transposon Tn6252
Autor: | Mohammad Hamidian, Steven J. Nigro, Ruth M. Hall, Rebecca M. Hartstein |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Microbiology (medical) Acinetobacter baumannii DNA Bacterial Imipenem Genomic Islands Tetracycline 030106 microbiology Context (language use) Microbial Sensitivity Tests Biology Microbiology beta-Lactamases 03 medical and health sciences Plasmid Antibiotic resistance Drug Resistance Multiple Bacterial medicine Humans Pharmacology (medical) Pharmacology Kanamycin Sequence Analysis DNA biology.organism_classification Anti-Bacterial Agents Transformation (genetics) Infectious Diseases Conjugation Genetic DNA Transposable Elements Tobramycin Gentamicins Genome Bacterial medicine.drug Acinetobacter Infections Plasmids |
Zdroj: | The Journal of antimicrobial chemotherapy. 72(7) |
ISSN: | 1460-2091 |
Popis: | © The Author 2017. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. Objectives: To determine the identity and context of genes conferring antibiotic resistance in a sporadic multiply antibiotic-resistant Acinetobacter baumannii recovered at Royal Children's Hospital, Brisbane. Methods: The antibiotic resistance phenotype for 23 antibiotics was determined using disc diffusion or MIC determination. The whole-genome sequence of RCH51 was determined using the Illumina HiSeq platform. Antibiotic resistance determinants were identified using ResFinder. Plasmids were recovered by transformation. Results: Isolate RCH51 belongs to the uncommon STs ST103IP (7-3-2-1-7-1-4) and ST514OX (1-52-29-28-18- 114-7). It was found to be resistant to sulfamethoxazole, tetracycline, gentamicin, tobramycin and kanamycin and also exhibited reduced susceptibility to imipenem(MIC 2 mg/L) and meropenem (MIC 6 mg/L). RCH51 carries the oxa235, sul2, floR, aadB and tet39 resistance genes, all located on plasmids. The largest of the three plasmids, pRCH51-3, is 52 789 bp and carries oxa235 in the ISAba1-bounded transposon Tn6252, as well as sul2 and floR. pRCH51-3 represents a new A. baumannii plasmid family that is potentially conjugative as it contains several genes predicted to encode transfer functions. However, conjugation of pRCH51-3 was not detected. The aadB and tet39 resistance genes were each found in small plasmids identical to the known plasmids pRAY*-v1 and pRCH52-1, respectively. Conclusions: The resistance gene complement of RCH51 was found in three plasmids. pRCH51-3, which carries the oxa235, sul2 and floR resistance genes, represents a new, potentially conjugative A. baumannii plasmid type. |
Databáze: | OpenAIRE |
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