Evaluating the efficiency of enzyme accelerated CO2 capture: chemical kinetics modelling for interpreting measurement results
| Autor: | Sonia Del Prete, Ada Fort, Marco Mugnaini, Claudiu T. Supuran, Clemente Capasso, Anna Lo Grasso, Valerio Vignoli, Lorenzo Parri, Maria Novella Romanelli |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Work (thermodynamics)
Bicarbonate Kinetics carbonic anhydrase Analytical chemistry chemistry.chemical_element Chemical RM1-950 01 natural sciences Chemical kinetics chemistry.chemical_compound chemical system measurement Gas measurement system kinetics modelling Bioreactors Carbon Dioxide Carbonic Anhydrases Hydrogen-Ion Concentration Models Chemical Models Gas measurement system chemical system measurement carbonic anhydrase kinetics modelling Phase (matter) Drug Discovery Bioreactor Pharmacology 010405 organic chemistry System of measurement Brief Report General Medicine Nitrogen 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry Therapeutics. Pharmacology |
| Zdroj: | Journal of Enzyme Inhibition and Medicinal Chemistry article-version (VoR) Version of Record Journal of Enzyme Inhibition and Medicinal Chemistry, Vol 36, Iss 1, Pp 393-400 (2021) |
| ISSN: | 1475-6374 1475-6366 |
| Popis: | In this paper, the efficiency of the carbonic anhydrase (CA) enzyme in accelerating the hydration of CO2 is evaluated using a measurement system which consists of a vessel in which a gaseous flow of mixtures of nitrogen and CO2 is bubbled into water or water solutions containing a known quantity of CA enzyme. The pH value of the solution and the CO2 concentration at the measurement system gas exhaust are continuously monitored. The measured CO2 level allows for assessing the quantity of CO2, which, subtracted from the gaseous phase, is dissolved into the liquid phase and/or hydrated to bicarbonate. The measurement procedure consists of inducing a transient and observing and modelling the different kinetics involved in the steady-state recovery with and without CA. The main contribution of this work is exploiting dynamical system theory and chemical kinetics modelling for interpreting measurement results for characterising the activity of CA enzymes. The data for model fitting are obtained from a standard bioreactor, in principle equal to standard two-phase bioreactors described in the literature, in which two different techniques can be used to move the process itself away from the steady-state, inducing transients. |
| Databáze: | OpenAIRE |
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