Protective effect of diarylheptanoids fromCurcuma comosaon primary rat hepatocytes againstt-butyl hydroperoxide-induced toxicity
| Autor: | Chadanat Noonin, Surawat Jariyawat, Apichart Suksamrarn, Kanoknetr Suksen, Pawinee Piyachaturawat, Watcharaporn Devakul Na Ayutthaya, Tumnoon Charaslertrangsi, Patoomratana Tuchinda |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine Stereochemistry Pharmaceutical Science Curcuma comosa Protective Agents 01 natural sciences Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Curcuma tert-Butylhydroperoxide Diarylheptanoids Drug Discovery medicine Animals Rats Wistar Pharmacology Liver injury Dose-Response Relationship Drug Traditional medicine biology Plant Extracts General Medicine medicine.disease biology.organism_classification Rats 0104 chemical sciences 010404 medicinal & biomolecular chemistry 030104 developmental biology Complementary and alternative medicine chemistry Hepatoprotection Toxicity Hepatocytes Molecular Medicine Zingiberaceae Lipid Peroxidation |
| Zdroj: | Pharmaceutical Biology. 54:853-862 |
| ISSN: | 1744-5116 1388-0209 |
| DOI: | 10.3109/13880209.2015.1088550 |
| Popis: | Curcuma comosa Roxb. (Zingiberaceae) has traditionally been used as an anti-inflammatory agent in liver, and recent study has shown its hepatoprotective effect against CCl4-induced liver injury in vivo.This study further assesses the protective effect of C. comosa extracts and its isolated compounds against tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity in isolated primary rat hepatocytes.Isolated primary hepatocytes were pretreated with either ethanol (5-50 μg/ml) or hexane extract (1-50 μg/ml), or two diarylheptanoids (4-35 μM): compound D-91 [1-(4-hydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-ol] and compound D-92 [(3S)-1-(3,4-dihydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-ol], from C. comosa for 2 h prior to exposure to 1.5 mM t-BHP for 15 and 30 min. Their hepatoprotective activities were then determined.t-BHP markedly caused the formation of MDA and ALT leakage from the hepatocytes. Pretreatment with the C. comosa ethanol extract showed greater protective effect than the hexane extract, and the effect was concentration related. Treating the hepatocytes with compound D-92 provided greater protective effect than compound D-91. IC50 values of compounds D-91, D-92, and silymarin for the protection of ALT leakage at 30 min were 32.7 ± 1.1, 9.8 ± 0.7, and 160 ± 8 μM, respectively. Further investigation showed that compound D-92 was more effective in maintaining the intracellular glutathione content in the t-BHP treated group, whereas the reduction in antioxidant enzymes, glutathione peroxidase and glutathione-S-transferase activities, were not improved.Results suggest that diarylheptanoids are the active principles that provide protection against t-BHP-induced injury. Their ability to maintain intracellular glutathione content is the main mechanisms underlying the protective action. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|