Chip electrophoresis with mass spectrometric detection in record speed
| Autor: | Detlev Belder, Stefanie Fritzsche, Peter Hoffmann |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Electrophoresis
Analyte Time Factors Spermidine Chemistry Microfluidics Biomedical Engineering Analytical chemistry Bioengineering General Chemistry Microfluidic Analytical Techniques Arginine Mass spectrometry Chip Niacin Biochemistry Mass Spectrometry Time of flight Data acquisition Mass spectrum Common emitter |
| Zdroj: | Lab on a Chip. 10:1227 |
| ISSN: | 1473-0189 1473-0197 |
| Popis: | We demonstrate that the combination of high speed separations on chip with a fast mass spectrometer enables electrophoretic separations with full mass spectra registration within a second. This was accomplished by coupling a microfluidic glass chip with an integrated nanospray emitter to a fast time of flight mass spectrometer working at 100 Hz for data acquisition. Applying field strengths up to 5800 V cm(-1) we achieved separations of model analytes such as pharmaceuticals and peptides with subsequent acquisition of full mass spectra within one second or slightly more. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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