Inhibin Subunits mRNA Expression Level in Human Placenta from Normal and Down's Syndrome Pregnancies
| Autor: | Karl Thomas, S. Pampfer, Frédéric Debiève |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
endocrine system medicine.medical_specialty endocrine system diseases Enzyme-Linked Immunosorbent Assay Gestational Age Ovary Biology Pregnancy Fetal membrane Placenta Internal medicine Gene expression medicine Humans Inhibins RNA Messenger Northern blot reproductive and urinary physiology G alpha subunit Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Glyceraldehyde-3-Phosphate Dehydrogenases Obstetrics and Gynecology Oligonucleotides Antisense Blotting Northern medicine.disease female genital diseases and pregnancy complications Trophoblasts medicine.anatomical_structure Endocrinology Reproductive Medicine Female Down Syndrome hormones hormone substitutes and hormone antagonists Developmental Biology |
| Zdroj: | Placenta. 22:96-102 |
| ISSN: | 0143-4004 |
| Popis: | In order to study the mechanisms leading to increased inhibin A and activin A in maternal serum with advancing gestation and increased inhibin A in Down's syndrome pregnancy, the mRNA expression level of inhibin and activin subunits was quantitatively studied in human placenta using Northern blot and semiquantitative RT-PCR analysis. The corresponding protein level was also determined by specific ELISAs for inhibin A, inhibin B, activin A and inhibin pro alphaC in placental extracts. Normal placenta (n=27) showed a slight significant increase in alpha and betaA subunit mRNA levels in term pregnancy, with no change of the corresponding protein level. Placenta from Down's syndrome pregnancies (n=6) did not differ from controls in either mRNA expression or corresponding protein levels. In conclusion, there is a dissociation between inhibin and activin subunit mRNA levels and the corresponding protein levels in maternal serum, and Down's syndrome inhibin A increase is not explained by mRNA expression upregulation. In an additional study, ovarian cortex tissue from term pregnancies (n=3) were examined. Only the alpha subunit mRNA was expressed, at a higher level than in the placenta, suggesting that ovary could be a source of inhibin pro alphaC during pregnancy. |
| Databáze: | OpenAIRE |
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