Visualizing Macrophage Extracellular Traps Using Confocal Microscopy
| Autor: | Stephen R. Holdsworth, Philip G. Bardin, Roleen Sharma, Paul T. King, Kim M. O’Sullivan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Extracellular Traps Proteases General Chemical Engineering Immunology Biology General Biochemistry Genetics and Molecular Biology law.invention Mice 03 medical and health sciences In vivo Confocal microscopy law Microscopy Extracellular Animals Humans Lung Mice Inbred BALB C Microscopy Confocal General Immunology and Microbiology Macrophages General Neuroscience Neutrophil extracellular traps Chromatin Cell biology Mice Inbred C57BL 030104 developmental biology |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/56459 |
| Popis: | A primary method used to define the presence of neutrophil extracellular traps (NETs) is confocal microscopy. We have modified established confocal microscopy methods to visualize macrophage extracellular traps (METs). These extracellular traps are defined by the presence of extracellular chromatin with co-expression of other components such as granule proteases, citrullinated histones, and peptidyl arginase deiminase (PAD). The expression of METs is generally measured after exposure to a stimulus and compared to un-stimulated samples. Samples are also included for background and isotype control. Cells are analyzed using well-defined image analysis software. Confocal microscopy may be used to define the presence of METs both in vitro and in vivo in lung tissue. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|