Mucin dispersions as a model for the oromucosal mucus layer in in vitro and ex vivo buccal permeability studies of small molecules
| Autor: | Mette Dalskov Mosgaard, Jette Bredahl Jacobsen, Eva Marxen, Anne Marie Lynge Pedersen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Saliva
Nicotine Barrier Buccal drug delivery Pharmaceutical Science 02 engineering and technology Biology 030226 pharmacology & pharmacy Epithelium Permeability Cell Line Absorption Diffusion Small Molecule Libraries Drug diffusion 03 medical and health sciences 0302 clinical medicine Caffeine medicine Humans Mannitol Oral mucosa Gastric Mucins Mucin Small molecules Mouth Mucosa General Medicine Buccal administration 021001 nanoscience & nanotechnology Mucus Molecular biology Propranolol medicine.anatomical_structure Biochemistry 0210 nano-technology Ex vivo Biotechnology medicine.drug |
| Zdroj: | Marxen, E, Mosgaard, M D, Pedersen, A M L & Jacobsen, J 2017, ' Mucin dispersions as a model for the oromucosal mucus layer in in vitro and ex vivo buccal permeability studies of small molecules ', European Journal of Pharmaceutics and Biopharmaceutics, vol. 121, pp. 121-128 . https://doi.org/10.1016/j.ejpb.2017.09.016 |
| Popis: | The mucus layer is believed to play a part in drug permeation across the oral mucosa. Human freeze-dried saliva (HFDS) and porcine gastric mucin (PGM) was evaluated as model for mucus layer per se or in conjunction with in vitro and ex vivo buccal permeability models. Four small molecules (nicotine, mannitol, propranolol, caffeine) showed decreased permeability across mucin dispersions, compared to controls, and a greater effect was seen with HFDS than with PGM. Permeability of propranolol and caffeine across filter-grown TR146 cells was decreased by the presence of mucin, whereas no effect was found on nicotine and mannitol. Incubation of porcine buccal mucosa with mucin dispersions for 24 h compromised the integrity of the tissue, whereas 30 min incubation did not affect tissue integrity. Tissue incubation with mucin dispersions did not decrease nicotine permeability. For the studied model drugs, it is concluded that mucin dispersions constitute a minor barrier for drug diffusion compared to the epithelium. |
| Databáze: | OpenAIRE |
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