Theranostic multimodular potential of zinc-doped ferrite-saturated metal-binding protein-loaded novel nanocapsules in cancers
| Autor: | Sishir K Kamalapuram, Rakesh Sehgal, Rupinder K. Kanwar, Rajneesh Chaudhary, Kislay Roy, Jagat R. Kanwar |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Pathology
Fluorescence-lifetime imaging microscopy Colorectal cancer theranostic Pharmaceutical Science Triple Negative Breast Neoplasms 02 engineering and technology Ferric Compounds Theranostic Nanomedicine Mice 0302 clinical medicine International Journal of Nanomedicine Drug Discovery Tumor Cells Cultured Original Research 2. Zero hunger real-time imaging biology Lactoferrin nanocapsules General Medicine 021001 nanoscience & nanotechnology nanomedicine 3. Good health Zinc 030220 oncology & carcinogenesis Colonic Neoplasms Female 0210 nano-technology medicine.medical_specialty Biophysics Mice Nude Bioengineering Adenocarcinoma Nanocapsules multimodular cancer therapy Biomaterials 03 medical and health sciences Breast cancer In vivo medicine Animals Humans business.industry Organic Chemistry medicine.disease Xenograft Model Antitumor Assays Apoptosis biology.protein Cancer research Cattle business Carrier Proteins Ex vivo |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Sishir K Kamalapuram,1 Rupinder K Kanwar,1 Kislay Roy,1 Rajneesh Chaudhary,1 Rakesh Sehgal,2 Jagat R Kanwar1 1Nanomedicine Laboratory of Immunology and Molecular Biomedical Research, School of Medicine, Centre for Molecular and Medical Research, Faculty of Health, Deakin University, Geelong, VIC, Australia; 2Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India Abstract: The present study successfully developed orally deliverable multimodular zinc (Zn) iron oxide (Fe3O4)-saturated bovine lactoferrin (bLf)-loaded polymeric nanocapsules (NCs), and evaluated their theranostic potential (antitumor efficacy, magnetophotothermal efficacy and imaging capability) in an in vivo human xenograft CpG-island methylator phenotype (CIMP)-1+/CIMP2−/chromosome instability-positive colonic adenocarcinoma (Caco2) and claudin-low, triple-negative (ER−/PR−/HER2−; MDA-MB-231) breast cancer model. Mice fed orally on the Zn-Fe-bLf NC diet showed downregulation in tumor volume and complete regression in tumor volume after 45 days of feeding. In human xenograft colon cancer, vehicle-control NC diet-group (n=5) mice showed a tumor volume of 52.28±11.55 mm3, and Zn-Fe-bLf NC diet (n=5)-treated mice had a tumor-volume of 0.10±0.073 mm3. In the human xenograft breast cancer model, Zn-Fe-bLf NC diet (n=5)-treated mice showed a tumor volume of 0.051±0.062mm3 within 40 days of feeding. Live mouse imaging conducted by near-infrared fluorescence imaging of Zn-Fe-bLf NCs showed tumor site-specific localization and regression of colon and breast tumor volume. Ex vivo fluorescence-imaging analysis of the vital organs of mice exhibited sparse localization patterns of Zn-Fe-bLf NCs and also confirmed tumor-specific selective localization patterns of Zn-Fe-bLf NCs. Dual imaging using magnetic resonance imaging and computerized tomography scans revealed an unprecedented theranostic ability of the Zn-Fe-bLf NCs. These observations warrant consideration of multimodular Zn-Fe-bLf NCs for real-time cancer imaging and simultaneous cancer-targeted therapy. Keywords: theranostic, multimodular cancer therapy, nanocapsules, nanomedicine, real-time imaging |
| Databáze: | OpenAIRE |
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