A rapid and sensitive method for the detection of histone peptides
| Autor: | Dennis J. O'Kane, Raymond F. Jones, Robert Levenson |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Biophysics
Xenopus Peptide Fluorescamine Biochemistry Histones chemistry.chemical_compound Methods medicine Molecular Biology Phthalaldehyde chemistry.chemical_classification Chromatography biology Cell Biology biology.organism_classification Oocyte Electrophoresis Histone medicine.anatomical_structure chemistry biology.protein Indicators and Reagents Peptides Digestion |
| Zdroj: | Analytical Biochemistry. 86:214-219 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(78)90336-6 |
| Popis: | Fluorescamine has been used to obtain a peptide map of a mixture of histones (H 3 , H 2 A, H 2 B, and H 4 ) prepared from oocytes of Xenopus laevis . Fluorescamine was found to be more sensitive than o -phthalaldehyde or ninhydrin-Cd for the detection of peptide fragments obtained from tryptic digestion of oocyte histones of X. laevis and the peptic digestion of the β chain of insulin. Using the β chain of insulin for a comparison, the 8 major peptide fragments could be separated by electrophoresis within 30 min and were detectable at the picomols level. Some 70 peptide spots of X. laevis oocyte histones were resolved, thus permitting the analysis of this complex mixture of polypeptides without the need for prior separation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|