Dach1 regulates neural crest migration during embryonic development
| Autor: | Tayaba Ismail, Hyun-Shik Lee, Youni Kim, Hongchan Lee, Yoo-Kyung Kim |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Morpholino Dachshund Biophysics Xenopus Embryonic Development Cell fate determination Biochemistry Xenopus laevis 03 medical and health sciences 0302 clinical medicine Animals Molecular Biology biology Embryogenesis Gene Expression Regulation Developmental Neural crest Cell Biology biology.organism_classification Cell biology 030104 developmental biology Neurula Neural Crest 030220 oncology & carcinogenesis Microcephaly Neural development Gene Deletion |
| Zdroj: | Biochemical and Biophysical Research Communications. 527:896-901 |
| ISSN: | 0006-291X |
| Popis: | Dachshund 1(Dach1) is a key component of the retinal determination gene network that plays significant roles in cell fate regulation. The vertebrate homolog of Drosophila dachshund has gained considerable importance as an essential regulator of development, but its functions during embryonic development remain elusive. We investigated the functional significance of dach1 during Xenopus embryogenesis using loss-of-function studies. Reverse transcription-polymerase chain reaction demonstrated the maternal nature of dach1, showing enhanced expression at the neurula stage of development, and morpholino oligonucleotide injection of dach1 induced phenotypic anomalies of microcephaly and reduced body length. Animal cap assays followed by whole-mount in-situ hybridization indicated the perturbed expression of neural and neural crest (NC) markers. Our data suggest the prerequisite functions of dach1 in NC migration during Xenopus embryogenesis. However, the developmental pathways regulated by dach1 during embryogenesis require further elucidation. |
| Databáze: | OpenAIRE |
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