Transcriptomic analysis of Citrus clementina mandarin fruits maturation reveals a MADS-box transcription factor that might be involved in the regulation of earliness
| Autor: | Manuel Talon, M. José Nueda, Francisco R. Tadeo, Daniel Ventimilla, Javier Terol |
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| Přispěvatelé: | Universidad de Alicante. Departamento de Matemáticas, Bioquímica Aplicada/Applied Biochemistry (AppBiochem), Sistemas Dinámicos y Estadística (SISDINEST) |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Citrus Mutant RNA-Seq MADS Domain Proteins Plant Science Biology Genes Plant 01 natural sciences Transcriptome 03 medical and health sciences Quantitative Trait Heritable Gene Expression Regulation Plant lcsh:Botany Estadística e Investigación Operativa Gene expression MADS box Gene Transcription factor Lateness MADS-box Plant Proteins Genetics Gene Expression Profiling Earliness food and beverages Ripening lcsh:QK1-989 030104 developmental biology Fruit 010606 plant biology & botany Research Article |
| Zdroj: | BMC Plant Biology RUA. Repositorio Institucional de la Universidad de Alicante Universidad de Alicante (UA) BMC Plant Biology, Vol 19, Iss 1, Pp 1-20 (2019) |
| ISSN: | 1471-2229 |
| Popis: | Background: Harvest time is a relevant economic trait in citrus, and selection of cultivars with different fruit maturity periods has a remarkable impact in the market share. Generation of early- and late-maturing cultivars is an important target for citrus breeders, therefore, generation of knowledge regarding the genetic mechanisms controlling the ripening process and causing the early and late phenotypes is crucial. In this work we analyze the evolution of the transcriptome during fruit ripening in 3 sport mutations derived from the Fina clementine (Citrus clementina) mandarin: Clemenules (CLE), Arrufatina (ARR) and Hernandina (HER) that differ in their harvesting periods. CLE is considered a mid-season cultivar while ARR and HER are early- and late-ripening mutants, respectively. Results: We used RNA-Seq technology to carry out a time course analysis of the transcriptome of the 3 mutations along the ripening period. The results indicated that in these mutants, earliness and lateness during fruit ripening correlated with the advancement or delay in the expression of a set of genes that may be implicated in the maturation process. A detailed analysis of the transcription factors known to be involved in the regulation of fruit ripening identified a member of the MADS box family whose expression was lower in ARR, the early-ripening mutant, and higher in HER, the late-ripening mutant. The pattern of expression of this gene during the maturation period was basically contrary to those of the ethylene biosynthetic genes, SAM and ACC synthases and ACC oxidase. The gene was present in hemizygous dose in the early-ripening mutant. Conclusions: Our analysis provides new clues about the genetic control of fruit ripening in citrus and allowed the identification of a transcription factor that could be involved in the early phenotype. Financial support was provided by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, INIA, (Ministerio de Economía, Industria y Competitividad e Innovación, Spain) trough grant # RTA-00071-C06–01. |
| Databáze: | OpenAIRE |
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