The Concurrent Detection of Chelonid Alphaherpesvirus 5 and Chelonia mydas Papillomavirus 1 in Tumoured and Non-Tumoured Green Turtles
| Autor: | Wytamma Wirth, Graham Burgess, Karina Jones, Ellen Ariel, Narges Mashkour |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Fibropapillomatosis
040301 veterinary sciences Chelonid alphaherpesvirus 5 Physiology Molecular evidence Biology Group A Group B Benign tumours Article law.invention 0403 veterinary science 03 medical and health sciences law lcsh:Zoology fibropapillomatosis biochemistry marine turtles lcsh:QL1-991 Turtle (robot) Chelonia mydas papillomavirus 1 030304 developmental biology 0303 health sciences lcsh:Veterinary medicine General Veterinary tumour 04 agricultural and veterinary sciences lcsh:SF600-1100 Animal Science and Zoology Viral load |
| Zdroj: | Animals, Vol 11, Iss 697, p 697 (2021) Animals : an Open Access Journal from MDPI Animals Volume 11 Issue 3 |
| ISSN: | 2076-2615 |
| Popis: | Characterised by benign tumours, fibropapillomatosis (FP) is a debilitating disease that predominantly afflicts the endangered green turtle (Chelonia mydas). A growing body of histological and molecular evidence has associated FP tumours with Chelonid alphaherpesvirus 5 (ChHV5). However, a recent study which detected both ChHV5 and Chelonia mydas papillomavirus 1 (CmPV1) DNA in FP tumour tissues has challenged this hypothesis. The present study aimed to establish a probe-based qPCR to assess the wider prevalence of CmPV1 and co-occurrence with ChHV5 in 275 marine turtles foraging in waters adjacent to the east coast of Queensland, Australia: three categories: Group A (FP tumours), Group B (non-tumoured skin from FP turtles) and Group C (non-tumoured skin from turtles without FP). Concurrent detection of ChHV5 and CmPV1 DNA is reported for all three categories, where Group A had the highest rate (43.5%). ChHV5 viral loads in Group A were significantly higher than loads seen in Group B and C. This was not the case for CmPV1 where the loads in Group B were highest, followed by Group A. However, the mean CmPV1 load for Group A samples was not significantly different to the mean load reported from Group B or C samples. Collectively, these results pivot the way we think about FP as an infectious disease where two separate viruses may be at play. |
| Databáze: | OpenAIRE |
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