Proinflammatory response of canine trophoblasts to Brucella canis infection

Autor: Pablo C. Baldi, Mariana C. Ferrero, Andrea G. Fernández, M. Soledad Hielpos, Carlos A. Fossati
Rok vydání: 2017
Předmět:
0301 basic medicine
Embryology
Chemokine
Physiology
Neutrophils
Placenta
medicine.medical_treatment
lcsh:Medicine
Monocytes
White Blood Cells
Animal Cells
Pregnancy
Immune Physiology
Medicine and Health Sciences
Brucella Canis
lcsh:Science
Immune Response
reproductive and urinary physiology
Mammals
Innate Immune System
Phagocytes
Multidisciplinary
biology
Chemotaxis
Toll-Like Receptors
Trophoblast
Eukaryota
Trophoblasts
Cell Motility
Canis
Cytokine
medicine.anatomical_structure
Vertebrates
embryonic structures
Brucella canis
Cytokines
Female
Cellular Types
Chemokines
Research Article
Immune Cells
Immunology
CCL5
Proinflammatory cytokine
03 medical and health sciences
Dogs
Immune system
medicine
Animals
Ciencias Exactas
Secretion
Inflammation
Antigens
Bacterial
Blood Cells
Ciencias Veterinarias
lcsh:R
Organisms
Abortion
Biology and Life Sciences
Cell Biology
Molecular Development
biology.organism_classification
030104 developmental biology
CIENCIAS AGRÍCOLAS
Immune System
Amniotes
biology.protein
lcsh:Q
Blastocysts
purl.org/becyt/ford/4.3 [https]
Physiological Processes
purl.org/becyt/ford/4 [https]
Developmental Biology
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PLoS ONE
SEDICI (UNLP)
Universidad Nacional de La Plata
instacron:UNLP
PLoS ONE, Vol 12, Iss 10, p e0186561 (2017)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0186561
Popis: Brucella canis infection is an important cause of late-term abortion in pregnant bitches. The pathophysiological mechanisms leading to B. canis–induced abortion are unknown, but heavily infected trophoblasts are consistently observed. As trophoblasts responses to other pathogens contribute to placental inflammation leading to abortion, the aim of the present study was to characterize the cytokine response of canine trophoblasts to B. canis infection. To achieve this, trophoblasts isolated from term placenta of healthy female dogs were infected with B. canis, culture supernatants were harvested for cytokine determinations, and the load of intracellular viable B. canis was determined at different times post-infection. Additionally, cytokine responses were assessed in non-infected trophoblasts stimulated with conditioned media (CM) from B. canis-infected canine monocytes and neutrophils. Finally, cytokine response and bacteria replication were assessed in canine placental explants infected ex vivo. B. canis successfully infected and replicated in primary canine trophoblasts, eliciting an increase in IL-8 and RANTES (CCL5) secretion. Moreover, the stimulation of trophoblasts with CM from B. canis-infected monocytes and neutrophils induced a significant increase in IL-8, IL-6 and RANTES secretion. B. canis replication was confirmed in infected placental explants and the infection elicited an increased secretion of TNF-α, IL-8, IL-6 and RANTES. This study shows that canine trophoblasts produce proinflammatory cytokines in response to B. canis infection and/or to stimulation with factors produced by infected monocytes and neutrophils. These cytokines may contribute to placental inflammation leading to abortion in B. canis-infected pregnant bitches.
Instituto de Estudios Inmunológicos y Fisiopatológicos
Databáze: OpenAIRE
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