Identification and characterization of an interspersed repetitive DNA fragment in Plasmodium vivax with potential use for specific parasite detection
Autor: | Marcela Ferella, Eduardo A. Guarnera, Marta Cabrera, Jorge H. Labbé, Sergio O. Angel, Hernando A. del Portillo, Silvana Carnevale, Björn Andersson, Jorge Néstor Velásquez |
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Rok vydání: | 2003 |
Předmět: |
Immunology
Plasmodium vivax Interspersed repeat Immunoblotting Molecular Sequence Data Protozoan Proteins Sequence alignment Polymerase Chain Reaction law.invention Tandem repeat law Aedes parasitic diseases Malaria Vivax Animals Humans Amino Acid Sequence Cloning Molecular Repeated sequence Polymerase chain reaction Genetics Electrophoresis Agar Gel biology Base Sequence Hybridization probe General Medicine Interspersed Repetitive Sequences DNA Protozoan biology.organism_classification Virology Culex Infectious Diseases Parasitology DNA Probes Sequence Alignment |
Zdroj: | Experimental parasitology. 108(3-4) |
ISSN: | 0014-4894 |
Popis: | We cloned and characterized a Plasmodium vivax repeat element of 7872 bp named PvRE7.8. Several internal tandem repeats were found along the sequence. The repetitive nature of the PvRE7.8 element was confirmed by hybridization of a P. vivax YAC library. Based on the data bank analysis and the presence of two contiguous putative genes that may encode proteins related to DNA metabolism, PvRE7.8 could be considered an inactivated transposon-LINE element. By using Pv79 as probe or primers derived from Pv79-flanking sequences, P. vivax DNA Could be detected from whole blood and mosquito samples. We consider that the repeat element described here has potential for P. vivax malaria diagnosis and for epidemiological analysis of P. vivax transmission areas. |
Databáze: | OpenAIRE |
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