A Novel Radiation-Resistant Strain of Filobasidium sp. Isolated from the West Sea of Korea
| Autor: | Harinder Singh, Hyun-Pa Song, Ha Ram Kim, Sangyong Lim, Yong Sun Bahn, Dong-Ho Kim, Min Ho Joe, Jong Il Choi |
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| Rok vydání: | 2013 |
| Předmět: |
DNA Repair
Sequence analysis Saccharomyces cerevisiae Biology Applied Microbiology and Biotechnology Microbiology Ionizing radiation RNA Ribosomal 18S Cluster Analysis Seawater Irradiation DNA Fungal Phylogeny Radiation resistance Korea Microbial Viability Strain (chemistry) Basidiomycota Fatty Acids Temperature Genes rRNA RNA Fungal Sequence Analysis DNA General Medicine biology.organism_classification Yeast genomic DNA Gamma Rays DNA Damage Biotechnology |
| Zdroj: | Journal of Microbiology and Biotechnology. 23:1493-1499 |
| ISSN: | 1738-8872 1017-7825 |
| DOI: | 10.4014/jmb.1305.05054 |
| Popis: | A novel radiation-resistant Filobasidium sp. yeast strain was isolated from seawater. Along with this strain, a total of 656 yeast isolates were purified from seawater samples collected from three locations in the West Sea of Korea and assessed for their radiation tolerance. Among these isolates, five were found to survive a 5 kGy radiation dose. The most radiationresistant strain was classified as Filobasidium sp. based on 18S rDNA sequence analysis and hence was named Filobasidium RRY1 (Radiation-Resistant Yeast 1). RRY1 differed from F. elegans, which is closely related to RRY1, in terms of the optimal growth temperature and radiation resistance, and was resistant to high doses of γ-ionizing radiation (D10: 6-7 kGy). When exposed to a high dose of 3 kGy irradiation, the RRY1 cells remained intact and undistorted, with negligible cell death. When these irradiated cells were allowed to recover, the cells fully repaired their genomic DNA within 3 h of growth recovery. This is the first report in which a radiation-resistant response has been investigated at the physiological, morphological, and molecular levels in a strain of Filobasidium sp. |
| Databáze: | OpenAIRE |
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