Rapid, B Lymphoid-Restricted Engraftment Mediated by a Primitive Bone Marrow Subpopulation
| Autor: | L. Jeanne Pierce, Gerald J. Spangrude, William B. Slayton, Mariluz P. Mojica, Suzanne J. Pohlmann, A. Elena Searles, Scott S. Perry |
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| Rok vydání: | 2000 |
| Předmět: |
Myeloid
Lymphocyte Immunology B-Lymphocyte Subsets Bone Marrow Cells Cell Separation Biology Colony-Forming Units Assay Mice Mice Congenic Graft Enhancement Immunologic medicine Animals Immunology and Allergy Cell Lineage Progenitor cell Bone Marrow Transplantation Interleukin 3 Cell Differentiation Hematopoietic Stem Cells Molecular biology Mice Inbred C57BL Transplantation Proto-Oncogene Proteins c-kit Haematopoiesis medicine.anatomical_structure Lymphocyte Transfusion Radiation Chimera Thy-1 Antigens Bone marrow Stem cell |
| Zdroj: | The Journal of Immunology. 165:67-74 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.165.1.67 |
| Popis: | Utilizing multiparameter flow cytometry, we have defined a subset of bone marrow cells containing lymphoid-restricted differentiation potential after i.v. transplantation. Bone marrow cells characterized by expression of the Sca-1 and c-kit Ags and lacking Ags of differentiating lineages were segregated into subsets based on allele-specific Thy-1.1 Ag expression. Although hematopoietic stem cells were recovered in the Thy-1.1low subset as previously described, the Thy-1.1neg subset consisted of progenitor cells that preferentially reconstituted the B lymphocyte lineage after i.v. transplantation. Recipients of Thy-1.1neg cells did not survive beyond 30 days, presumably due to the failure of erythroid and platelet lineages to recover after transplants. Thy-1.1neg cells predominantly reconstituted the bone marrow and peripheral blood of lethally irradiated recipients with B lineage cells within 2 weeks, although a low frequency of myeloid lineage cells was also detected. In contrast, myeloid progenitors outnumbered lymphoid progenitors when the Thy-1.1neg population was assayed in culture. When Thy-1.1low stem cells were rigorously excluded from the Thy-1.1neg subset, reconstitution of T lymphocytes was rarely observed in peripheral blood after i.v. transplantation. Competitive repopulation studies showed that the B lymphoid reconstitution derived from Thy-1.1neg cells was not sustained over a 20-wk period. Therefore, the Thy-1.1neg population defined in these studies includes transplantable, non-self-renewing B lymphocyte progenitor cells. |
| Databáze: | OpenAIRE |
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