Cloning and expression of the M5 RNA segment encoding outer capsid VP5 of epizootic hemorrhagic disease virus Japan serotype 2, Ibaraki virus
| Autor: | Hiroyuki Iwata, Ryoko Tokunaga, Takeshi Inoue, Tomoko Nishida, Eva Megumi Nara Pereira |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Serotype
viruses Molecular Sequence Data Hemorrhagic Disease Virus Epizootic Biology Molecular cloning Spodoptera Mice Capsid Complementary DNA Animals Amino Acid Sequence Cloning Molecular Peptide sequence General Veterinary Base Sequence Sequence Homology Amino Acid Epizootic hemorrhagic disease virus Nucleic acid sequence RNA Virology Molecular biology Open reading frame RNA Viral Capsid Proteins Electrophoresis Polyacrylamide Gel Baculoviridae |
| Zdroj: | The Journal of veterinary medical science. 62(3) |
| ISSN: | 0916-7250 |
| Popis: | The complete nucleotide sequence of a cDNA clone representing the M5 RNA segment of epizootic hemorrhagic disease virus Japan serotype 2 (EHDV-2), Ibaraki virus, was determined. The M5 segment is 1641 base pairs long with the single open reading frame which predicts a polypeptide of 527 amino acids. The comparison of the amino acid sequence of the VP5 with those of EHDV-1, bluetongue virus serotype 10, and African horse sickness virus serotype 4 revealed that the protein shared 67%, 57% and 42% homologies, respectively. In addition, the VP5 protein was expressed in insect cells by recombinant baculovirus, which could be recognized by the mouse anti-EHDV-2 sera at a position of the expected 59 kDa on immunoblot analysis. |
| Databáze: | OpenAIRE |
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