Delayed cytotoxic and genotoxic effects in a human cell line following X-irradiation
| Autor: | Myrtill Simkó, K.-R. Trott, Ralf Kriehuber, Dietmar Schiffmann |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Pathology
medicine.medical_specialty Time Factors Cell Survival Binucleated cells Centromere Biology Radiation Dosage complex mixtures chemistry.chemical_compound Tumor Cells Cultured medicine Humans Cytotoxic T cell Radiology Nuclear Medicine and imaging Cytochalasin B In Situ Hybridization Fluorescence Micronuclei Chromosome-Defective Chromosome Aberrations Radiological and Ultrasound Technology Molecular biology Squamous carcinoma chemistry Epidermoid carcinoma Micronucleus test Micronucleus Cell Division |
| Zdroj: | Web of Science |
| ISSN: | 1362-3095 0955-3002 |
| Popis: | In order to clarify the relationship between delayed reproductive death and radiation-induced genomic instability, the colony-forming efficiency of surviving, irradiated human squamous carcinoma cells and centromere positive as well as centromere negative micronuclei in surviving progeny were examined.Colony-forming ability and micronucleus (MN) frequency in binucleated cells 24 h after the addition of cytochalasin B during 2 weeks of post-irradiation growth were determined in a squamous cell carcinoma cell line (SCL-II) of human origin. In addition, centromeres in micronuclei were detected using FISH.In the human epithelial cell line used for these experiments, delayed reproductive death was pronounced and persisted for at least 2 weeks after irradiation. Although there is evidence for an increased rate of centromere positive micronuclei, but not of centromere negative micronuclei, arising during the first week of post-irradiation proliferation, this decreases later while the rate of delayed reproductive death remains elevated.In the studied cell line, the observed delayed reproductive death is not closely related to the investigated criteria of radiation-induced genomic instability. This casts doubt on the common assumption that delayed reproductive death is a direct manifestation of radiation-induced genomic instability. |
| Databáze: | OpenAIRE |
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