Overexpression ofblaOXA-58Gene Driven by ISAba3Is Associated with Imipenem Resistance in a ClinicalAcinetobacter baumanniiIsolate from Vietnam
| Autor: | Son Cong Pham, Tuan Manh Ha, Chau Van Vinh Nguyen, Thanh Tri Nguyen, Tuan Anh Nguyen, Anh Kim Ly |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Imipenem Article Subject 030106 microbiology Biology General Biochemistry Genetics and Molecular Biology law.invention Microbiology 03 medical and health sciences law Gene expression medicine Agar diffusion test Gene Polymerase chain reaction General Immunology and Microbiology General Medicine Periplasmic space biochemical phenomena metabolism and nutrition Antimicrobial biology.organism_classification Acinetobacter baumannii Multiple drug resistance 030104 developmental biology Medicine medicine.drug |
| Zdroj: | BioMed Research International, Vol 2020 (2020) |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2020/7213429 |
| Popis: | The aim of this study was to investigate genetic structures and expression ofblaOXA-58gene in fiveAcinetobacter baumanniiclinical isolates recovered from two hospitals in southern Vietnam during 2012-2014.A. baumanniiisolates were identified by automated microbiology systems and confirmed by PCR. All isolates were characterized as multidrug resistant by antimicrobial testing using the disk diffusion method. Four imipenem susceptible and one nonsusceptible isolates (MIC>32 μg·ml-1) were identified by E-test. PCR amplification ofblaOXA-58gene upstream and downstream sequences revealed the presence of ISAba3at both locations in one multidrug-resistant isolate. Semiquantitation ofblaOXA-51andblaOXA-58gene expression was performed by the 2-ΔΔCtmethod. TheblaOXA-51gene expression of five isolates showed little difference, but the isolate bearing ISAba3-blaOXA-58-ISAba3exhibited significantly higherblaOXA-58mRNA level. Higherβ-lactamases activity in periplasmic than cytoplasmic fraction was found in most isolates. The isolate overexpressingblaOXA-58gene possessed very high periplasmic enzyme activity. In conclusion, theA. baumanniiisolate bearing ISAba3-blaOXA-58gene exhibited high resistance to imipenem, corresponding to an overexpression ofblaOXA-58gene and very high periplasmicβ-lactamase activity. |
| Databáze: | OpenAIRE |
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