Comparative Depigmentation Effects of Resveratrol and Its Two Methyl Analogues in α-Melanocyte Stimulating Hormone-Triggered B16/F10 Murine Melanoma Cells
| Autor: | Dong-Bum Shin, Nam-Ho Lee, Hoon-Seok Yoon, Chang-Gu Hyun, Sung-Soo Park |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
pterostilbene Pterostilbene Melanocyte-stimulating hormone Tyrosinase Resveratrol Biology tyrosinase methyl resveratrol depigmentation Melanin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine melan-A Hypopigmentation Nutrition and Dietetics integumentary system Arbutin Molecular biology Research Note 030104 developmental biology Pinostilbene chemistry Biochemistry 030220 oncology & carcinogenesis medicine.symptom hormones hormone substitutes and hormone antagonists Food Science |
| Zdroj: | Preventive Nutrition and Food Science |
| ISSN: | 2287-1098 |
| Popis: | Previous research showed that resveratrol (trans-3,4',5-trihydroxystilbene) and pinostilbene (trans-3-methoxy-4',5-dihydroxystilbene) were able to inhibit tyrosinase directly; however, anti-melanogenic effects of pterostilbene (trans-3,5-dimethoxy-4'-hydroxystilbene) and resveratrol trimethyl ether (RTE) have not been compared. To investigate the hypopigmentation effects of pterostilbene and RTE, melanin contents and intracellular tyrosinase activity were determined by western blot analysis. Firstly, pterostilbene showed the inhibitory effects on α-melanocyte stimulating hormone (MSH)-induced melanin synthesis stronger than RTE, resveratrol, and arbutin. Pterostilbene inhibited melanin biosynthesis in a dose-dependent manner in α-MSH-stimulated B16/F10 murine melanoma cells. Specifically, melanin content and intracellular tyrosinase activity were inhibited by 63% and 58%, respectively, in response to treatment with 10 μM of pterostilbene. The results of western blot analysis indicated that pterostilbene induced downregulation of tyrosinase protein expression and suppression of α-MSH-stimulated melan-A protein expression stronger than RTE or resveratrol. Based on these results, our study suggests that pterostilbene can induce hypopigmentation effects more effectively than resveratrol and RTE, and it functions via downregulation of protein expression associated with hyperpigmentation in α-MSH-triggered B16/F10 murine melanoma cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|