GROWTH FACTOR-INDUCED PROLIFERATIVE RESPONSES OF HUMAN AND DMH-INDUCED RAT COLORECTAL TUMOUR CELL LINES
| Autor: | Moira Maley, A. K. House, M. V. Agrez |
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| Rok vydání: | 1986 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.medical_treatment Clinical Biochemistry Immunology Tumor cells Biology Cell Line Species Specificity Epidermal growth factor Internal medicine medicine Animals Humans Tumor Stem Cell Assay Dimethylhydrazines Epidermal Growth Factor Rectal Neoplasms Cell growth Growth factor Colorectal tumour Rats Inbred Strains Cell Biology General Medicine In vitro 1 2-Dimethylhydrazine Rats Endocrinology Cell culture Colonic Neoplasms Cancer research Cell Division |
| Zdroj: | Australian Journal of Experimental Biology and Medical Science. 64:445-451 |
| ISSN: | 0004-945X |
| DOI: | 10.1038/icb.1986.47 |
| Popis: | The proliferative response of three human and three 1,2-dimethylhydrazine (DMH)-induced rat colon tumour cell lines to mouse epidermal growth factor (mEGF) and human urogastrone was examined in two in vitro assays (3H-thymidine uptake in liquid microculture and clonogenicity in semi-solid medium). In the 3H-thymidine uptake assay, one of three rat and one of three human colon tumour cell lines, i.e., r237 and LIM1215, were stimulated by mEGF. In the clonogenic assay, mEGF stimulated the one clonogenic rat tumour cell line r113, which did not respond in the 3H-thymidine uptake assay, and the human tumour cell line LIM1215. Urogastrone stimulated LIM1215 in both assay systems. The significance of these in vitro patterns of response to growth stimulatory factors is discussed. |
| Databáze: | OpenAIRE |
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