An electrochemical immunosensor for anti-T. cruzi IgM antibodies, a biomarker for congenital Chagas disease, using a screen-printed electrode modified with gold nanoparticles and functionalized with shed acute phase antigen
| Autor: | Pedro R. Aranda, Franco A. Bertolino, Carlos D. Garcia, Julio Raba, Sirley V. Pereira, Matías Regiart |
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| Rok vydání: | 2016 |
| Předmět: |
Chagas disease
SCANNING ELECTRON MICROSCOPY Recombinant antigen Igm antibody 030204 cardiovascular system & hematology 01 natural sciences Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Antigen RECOMBINANT ANTIGEN Medicine Sandwich immunoassay X-RAY DIFFRACTION business.industry Otras Ciencias Químicas Screen printed electrode SEROLOGY 010401 analytical chemistry Ciencias Químicas medicine.disease Molecular biology SANDWICH IMMUNOASSAY 0104 chemical sciences CYCLIC VOLTAMMETRY HORSERADISH PEROXIDASE business CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Microchimica Acta. 183:1203-1210 |
| ISSN: | 1436-5073 0026-3672 |
| DOI: | 10.1007/s00604-016-1752-4 |
| Popis: | The authors describe a sandwich immunosensor for anti-IgM T. cruzi-specific antibodies which are a biomarker for congenital Chagas disease (CCD). A screen-printed carbon electrode (SPCE) was modified with gold nanoparticles (AuNPs) and then functionalized with recombinant shed acute phase antigen (SAPA). The AuNPs/SPCE was characterized by scanning electron microscopy, cyclic voltammetry, and X-ray diffraction. The AuNPs provide an increased active surface area, high conductivity, and improved electrocatalytic characteristics, this resulting in a significant improvement of the detection limit. Following interaction between immobilized SAPA and the target antibodies in the serum sample, secondary antibody (an anti-human IgM-HRP conjugate) and solutions of H2O2 and 4-tert-butylcatechol were added and the enzymatic product was detected at −100 mV (vs. Ag/SPCE). The resulting current was proportional to the anti-T. cruzi IgM antibodies present in the sample and showed a linear trend from 10 to 200 ng mL−1 (R = 0.998). The detection limit was 3.03 ng mL−1. These values demonstrate that this electrochemical immunosensor can be used as an alternative serological method for diagnosis of congenital Chagas disease. Fil: Regiart, Daniel Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Garcia, Carlos D.. Clemson University; Estados Unidos Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Aranda, Pedro Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina |
| Databáze: | OpenAIRE |
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