Multichannel optogenetic stimulation of the auditory pathway using microfabricated LED cochlear implants in rodents
| Autor: | Christian Wrobel, Vladan Rankovic, Alexander Dieter, Tobias Moser, Christian Vogl, Gerhard Hoch, Michael Schwaerzle, Tamas Harczos, Lukasz Jablonski, Bettina Wolf, Daniel Keppeler, Oliver Paul, Patrick Ruther, Khaled Abdellatif, Suleman Ayub, Marcus Jeschke |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Auditory Pathways Materials science Channelrhodopsin Stimulation Optogenetics Article 03 medical and health sciences 0302 clinical medicine otorhinolaryngologic diseases medicine Animals Spiral ganglion Cochlea General Medicine Cochlear Implantation Electric Stimulation Rats Electrophysiology Cochlear Implants 030104 developmental biology medicine.anatomical_structure Auditory information sense organs Tonotopy Spiral Ganglion Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Sci Transl Med |
| ISSN: | 1946-6242 1946-6234 |
| DOI: | 10.1126/scitranslmed.abb8086 |
| Popis: | When hearing fails, electrical cochlear implants (eCIs) provide the brain with auditory information. One important bottleneck of CIs is the poor spectral selectivity that results from the wide current spread from each of the electrode contacts. Optical CIs (oCIs) promise to make better use of the tonotopic order of spiral ganglion neurons (SGNs) inside the cochlea by spatially confined stimulation. Here we established multichannel oCIs based on light-emitting diode (LED) arrays and used them for optical stimulation of channelrhodopsin (ChR)-expressing SGNs in rodents. Power-efficient blue LED chips were integrated onto microfabricated 15-μm-thin polyimide-based carriers comprising interconnecting lines to address individual LEDs by a stationary or mobile driver circuitry. We extensively characterized the optoelectronic, thermal, and mechanical properties of the oCIs and demonstrated stability over weeks in vitro. We then implanted the oCIs into ChR-expressing rats and gerbils and characterized multichannel optogenetic SGN stimulation by electrophysiological and behavioral experiments. Improved spectral selectivity was directly demonstrated by recordings from the auditory midbrain. Longterm experiments in deafened ChR-expressing rats and in non-treated control animals demonstrated specificity of optogenetic stimulation. Behavioral studies on animals carrying a wireless oCI sound processor revealed auditory percepts. In conclusion, the study demonstrates hearing restoration with improved spectral selectivity by a LED-based multichannel oCI system. |
| Databáze: | OpenAIRE |
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