Compact and tunable stretch bioreactor advancing tissue engineering implementation. Application to engineered cardiac constructs
| Autor: | Stefano Gabetti, Immacolata Belviso, Clotilde Castaldo, Dario Carbonaro, Anna Maria Sacco, Veronica Romano, Umberto Morbiducci, Diana Nada Caterina Massai, Franca Di Meglio, Gianpaolo Serino, Cristina Bignardi, Giovanni Putame |
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| Přispěvatelé: | Putame, G., Gabetti, S., Carbonaro, D., Di Meglio, F., Romano, V., Sacco, A. M., Belviso, I., Serino, G., Bignardi, C., Morbiducci, U., Castaldo, C., Massai, D. |
| Rok vydání: | 2020 |
| Předmět: |
Cyclic stretching
Cardiac progenitors Decellularization Mechanical stimulation Tissue Engineering Tissue Scaffolds Computer science 0206 medical engineering Biomedical Engineering Biophysics Bioreactor 02 engineering and technology 020601 biomedical engineering 03 medical and health sciences 0302 clinical medicine Cardiac tissue maturation Bioreactors Tissue engineering Humans Tissues engineering 030217 neurology & neurosurgery Bioreactor Cyclic stretching Mechanical stimulation Tissues engineering Cardiac tissue maturation Biomedical engineering |
| Zdroj: | Medical engineeringphysics. 84 |
| ISSN: | 1873-4030 |
| Popis: | Physical stimuli are crucial for the structural and functional maturation of tissues both in vivo and in vitro. In tissue engineering applications, bioreactors have become fundamental and effective tools for providing biomimetic culture conditions that recapitulate the native physical stimuli. In addition, bioreactors play a key role in assuring strict control, automation, and standardization in the production process of cell-based products for future clinical application. In this study, a compact, easy-to-use, tunable stretch bioreactor is proposed. Based on customizable and low-cost technological solutions, the bioreactor was designed for providing tunable mechanical stretch for biomimetic dynamic culture of different engineered tissues. In-house validation tests demonstrated the accuracy and repeatability of the imposed mechanical stimulation. Proof of concepts biological tests performed on engineered cardiac constructs, based on decellularized human skin scaffolds seeded with human cardiac progenitor cells, confirmed the bioreactor Good Laboratory Practice compliance and ease of use, and the effectiveness of the delivered cyclic stretch stimulation on the cardiac construct maturation. |
| Databáze: | OpenAIRE |
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