Changing pattern of prevalence and genetic diversity of rotavirus, norovirus, astrovirus, and bocavirus associated with childhood diarrhea in Asian Russia, 2009-2012
Autor: | Sergey Sokolov, Daria A. Sedelnikova, Alexander Tymentsev, Elena Zhirakovskaia, Nina V. Tikunova, Artem Tikunov |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Male Rotavirus viruses medicine.disease_cause Russia fluids and secretions Genotype Odds Ratio Prevalence Public Health Surveillance Child Phylogeny Age Factors virus diseases Diarrhea Infectious Diseases Child Preschool RNA Viral Female Seasons medicine.symptom Microbiology (medical) 030106 microbiology Biology Microbiology History 21st Century Virus Astrovirus Bocavirus 03 medical and health sciences Age Distribution Genetics medicine Humans Molecular Biology Genotyping Ecology Evolution Behavior and Systematics Genetic diversity Norovirus Genetic Variation biology.organism_classification Virology Molecular Typing 030104 developmental biology Astroviridae |
Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 67 |
ISSN: | 1567-7257 |
Popis: | This hospital-based surveillance study was carried out in Novosibirsk, Asian Russia from September 2009 to December 2012. Stool samples from 5486 children with diarrhea and from 339 healthy controls were screened for rotavirus, norovirus, astrovirus, and bocavirus by RT-PCR. At least one enteric virus was found in 2075 (37.8%) cases with diarrhea and 8 (2.4%) controls. In the diarrhea cases, rotavirus was the most commonly detected virus (24.9%), followed by norovirus (13.4%), astrovirus (2.8%) and bocavirus (1.1%). Mixed viral infections were identified in 4.3% cases. The prevalence of enteric viruses varied every season. Rotavirus infection was distributed in a typical seasonal pattern with a significant annual increase from November to May, while infections caused by other viruses showed no apparent seasonality. The most common rotavirus was G4P[8] (56%), followed by G1P[8] (20.1%), G3P[8] (5.5%), G9P[8], G2P[4] (each 1.3%), six unusual (1.2%), and five mixed strains (0.5%). Norovirus GII.3 (66.5%) was predominant, followed by GII.4 (27.3%), GII.6 (3.7%), GII.1 (1.6%), and four rare genotypes (totally, 0.9%). Re-infection with noroviruses of different genotypes was observed in four children. The classic human astrovirus belonged to HAstV-1 (82%), HAstV-5 (8%), HAstV-4 (4.7%), HAstV-3 (4%) and HAstV-2 (1.3%). Consecutive episodes of HAstV-1 and HAstV-4 infections were detected in one child with an 8-month interval. Bocavirus strains were genotyped as HBoV2 (56.5%), HBoV1 (38.7%), HBoV4 (3.2%) and HBoV3 (1.6%). In the controls, norovirus strains belonged to GII.4 (n = 4), GII.1, GII.3, and GII.6, and HBoV2 strain were detected. Most of the detected virus isolates were characterized by a partial sequencing of the genomes. The genotype distribution of most common enteric viruses found in the Asian part of Russia did not differ considerably from their distribution in European Russia in 2009–2012. |
Databáze: | OpenAIRE |
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