Enhanced Expression of The LDH-A Gene after Gravity-changing Stress in Human RSa Cells
| Autor: | Tetsuhiro Moriya, Nobuo Suzuki, Kazuko Kita, Chieko Wano, Shigeru Sugaya |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Lactate dehydrogenase A
Centrifugation Hypergravity Biology Cell Line Stress (mechanics) Gene expression Humans RNA Messenger Northern blot skin and connective tissue diseases education Gene Cells Cultured Differential display education.field_of_study L-Lactate Dehydrogenase Weightlessness General Medicine Space Flight Molecular biology Isoenzymes Gene Expression Regulation sense organs Lactate Dehydrogenase 5 Mrna differential display Gravitation |
| Zdroj: | Biological Sciences in Space. 16:12-17 |
| ISSN: | 1349-967X 0914-9201 |
| DOI: | 10.2187/bss.16.12 |
| Popis: | A major issue in radiation and space biology is whether gene expression levels are altered in cells exposed to gravity-changing stress. In the present study, genes up- or down-regulated in radiation-sensitive human RSa cells cultured under gravity-changing conditions, were identified using a PCR-based mRNA differential display method. Exposure of cells to gravity-changing stress was performed by free-fall with a drop-shaft facility or by an airplane-conducted parabolic flight. Among the candidates for gravity-changing stress-responsive genes obtained by the differential display analysis, the lactate dehydrogenase A gene (LDH-A) was confirmed by Northern blotting analysis to exhibit increased expression levels. The gravity-changing stress consisted of a combination of microgravity and hypergravity. However, exposure of the cells to hypergravity produced by centrifuge only slightly affected the LDH-A mRNA expression. Thus, LDH-A was found to be a candidate for the genes which play a role in the cellular response to gravity-changing stress, and mainly to microgravity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|