Worsening of obesity and metabolic status yields similar molecular adaptations in human subcutaneous and visceral adipose tissue: decreased metabolism and increased immune response
| Autor: | Nathalie Viguerie, Vladimir Stich, Petra Šrámková, Michaela Siklova-Vitkova, Anne Bouloumié, Dominique Langin, Zuzana Kovacova, Eva Klimcakova, Adriana Márquez-Quiñones, Balbine Roussel, Jindra Hejnova, Michaela Kovacikova, Marion Combes |
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| Přispěvatelé: | Franco-czech Laboratory for clinical research on obesity, Charles University [Prague] (CU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Fédératif de Recherche 150, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, OB Clinic, AVENIR team 'Vascular network, progenitor cells and immune cells', Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-IFR150-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Laboratoire de Biochimie, CHU Toulouse [Toulouse]-Institut de Biologie de Purpan, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Laboratoire de Biochimie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Simon, Marie Francoise |
| Rok vydání: | 2010 |
| Předmět: |
Endocrinology
Diabetes and Metabolism Clinical Biochemistry Adipose tissue Gene Expression Overweight Biochemistry MESH: Glucose Clamp Technique MESH: Down-Regulation 0302 clinical medicine Endocrinology MESH: Metabolic Syndrome X MESH: Obesity MESH: Aged Metabolic Syndrome 0303 health sciences MESH: Middle Aged Glucose clamp technique Middle Aged MESH: Insulin Resistance Female medicine.symptom Adult medicine.medical_specialty MESH: Gene Expression Subcutaneous Fat Down-Regulation 030209 endocrinology & metabolism Context (language use) Biology Intra-Abdominal Fat 03 medical and health sciences Insulin resistance MESH: Subcutaneous Fat Internal medicine medicine Humans Obesity MESH: Intra-Abdominal Fat 030304 developmental biology Aged MESH: Humans Biochemistry (medical) nutritional and metabolic diseases MESH: Adult medicine.disease Gene expression profiling Glucose Clamp Technique Metabolic syndrome Insulin Resistance MESH: Female |
| Zdroj: | Journal of Clinical Endocrinology and Metabolism Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 2011, 96 (1), pp.E73-82. ⟨10.1210/jc.2010-1575⟩ Journal of Clinical Endocrinology and Metabolism, 2011, 96 (1), pp.E73-82. ⟨10.1210/jc.2010-1575⟩ |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jc.2010-1575⟩ |
| Popis: | Context: It is not known whether biological differences reported between sc adipose tissue (SAT) and visceral adipose tissue (VAT) depots underlie the pathogenicity of visceral fat. Objective: We compared SAT and VAT gene expression according to obesity, visceral fat accumulation, insulin resistance, and presence of the metabolic syndrome. Design: Subjects were assigned into four groups (lean, overweight, obese, and obese with metabolic syndrome). Setting: Subjects were recruited at a university hospital. Patients: Thirty-two women were included. Main Outcome Measures: Anthropometric measurements, euglycemic-hyperinsulinemic clamps, blood analyses, and computed tomography scans were performed, and paired samples of SAT and VAT were obtained for DNA microarray-based gene expression profiling. Results: Considering the two fat depots together, 1125 genes were more and 1025 genes were less expressed in lean compared with metabolic syndrome subjects. Functional annotation clustering showed, from lean to metabolic syndrome subjects, progressive down-regulation of metabolic pathways including branched-chain amino acid, fatty acid, carbohydrate, and mitochondrial energy metabolism and up-regulation of immune response genes involved in toll-like receptor, TNF, nuclear factor-κB, and apoptosis pathways. Metabolism and immune response genes showed an opposite correlation with fat mass, fat distribution, or insulin resistance indices. These associations were similar in SAT and VAT, although about 1000 genes showed differential expression between SAT and VAT. Conclusions: The increase in adiposity and the worsening of metabolic status are associated with a coordinated down-regulation of metabolism-related and up-regulation of immune response-related gene expression. Molecular adaptations in SAT prove as discriminating as those in VAT. |
| Databáze: | OpenAIRE |
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