Targeting VEGF with LNA-stabilized G-rich oligonucleotide for efficient breast cancer inhibition

Autor:	Stacey L. Edwards, Michael B. Petersen, Kristine M. Hillman, Vasanthanathan Poongavanam, Maja Marušič, Janez Plavec, Rikke Leth-Larsen, Rakesh N. Veedu, Kislay Roy, Jesper Wengel, Jagat R. Kanwar, Neerati Prasad
Rok vydání:	2015
Předmět:	Vascular Endothelial Growth Factor A Aptamers Nucleotide/chemistry Guanine Aptamer Oligonucleotides Mice Nude Cell Movement/drug effects Antineoplastic Agents Breast Neoplasms Human Umbilical Vein Endothelial Cells/drug effects Molecular Dynamics Simulation Catalysis Cell Proliferation/drug effects chemistry.chemical_compound Breast cancer Breast Neoplasms/drug therapy In vivo Cell Movement Cell Line Tumor Antineoplastic Agents/chemistry Materials Chemistry medicine Human Umbilical Vein Endothelial Cells Animals Humans Nucleotide Locked nucleic acid Oligonucleotides/chemistry Cell Proliferation chemistry.chemical_classification Oligonucleotide Chemistry Cell growth Metals and Alloys Vascular Endothelial Growth Factor A/metabolism General Chemistry Aptamers Nucleotide medicine.disease Molecular biology Surfaces Coatings and Films Electronic Optical and Magnetic Materials Tumor Burden Molecular Docking Simulation Ceramics and Composites Cancer research Female Tumor Burden/drug effects DNA
Zdroj:	Edwards, S L, Poongavanam, V, R Kanwar, J, Roy, K, M. Hillman, K, Prasad, N, Leth-Larsen, R, Petersen, M, Marusic, M, Plavec, J, Wengel, J & Veedu, R N 2015, ' Targeting VEGF with LNA-stabilized G-rich oligonucleotide for efficient breast cancer inhibition ', Chemical Communications, vol. 51, no. 46, pp. 9499-9502 . https://doi.org/10.1039/c5cc02756j
ISSN:	1364-548X
DOI:	10.1039/c5cc02756j
Popis:	In this study, we investigated the efficacy of an LNA (locked nucleic acid)-modified DNA aptamer named RNV66 targeting VEGF against various breast cancer cell lines. Our results demonstrate that RNV66 efficiently inhibits breast cancer cell proliferation both in vitro and in vivo. Introduction of LNA nucleotides were crucial for higher efficacy. Furthermore, the binding interaction of RNV66 with VEGF was investigated using molecular dynamic simulations leading to the first computational model of the LNA aptamer-VEGF complex blocking its interaction with VEGF-receptor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04fe8e33bcaade89338e5c0441d9769e https://pubmed.ncbi.nlm.nih.gov/25968110 Zobrazit plný text záznamu