Transcriptional regulation of post-aggregation genes in Dictyostelium by a feed-forward loop involving GBF and LagC

Autor: Negin Iranfar, William F. Loomis, Danny Fuller
Rok vydání: 2005
Předmět:
Transcriptional profiling
DNA
Complementary
Transcription
Genetic
Mutant
Amino Acid Motifs
Models
Biological
Post-aggregative genes
Gene expression
Cell Adhesion
Animals
Dictyostelium
Genes
Developmental
Gene
Adhesion signaling
Molecular Biology
Oligonucleotide Array Sequence Analysis
Regulation of gene expression
Genetics
Dictyostelium development
biology
Models
Genetic
Gene Expression Profiling
Wild type
Computational Biology
Gene Expression Regulation
Developmental
Membrane Proteins
Cell Biology
DNA
Genomics
biology.organism_classification
Blotting
Northern
DNA binding protein
Gene expression profiling
DNA-Binding Proteins
G-Box Binding Factors
Gene Expression Regulation
Mutation
Feed-forward loop
RNA
DNA microarray
Developmental Biology
Adenylyl Cyclases
Protein Binding
Signal Transduction
Zdroj: Developmental biology. 290(2)
ISSN: 0012-1606
Popis: Expression profiles of developmental genes in Dictyostelium were determined on microarrays during development of wild type cells and mutant cells lacking either the DNA binding protein GBF or the signaling protein LagC. We found that the mutant strains developed in suspension with added cAMP expressed the pulse-induced and early adenylyl cyclase (ACA)-dependent genes, but not the later ACA-dependent, post-aggregation genes. Since expression of lagC itself is dependent on GBF, expression of the post-aggregation genes might be controlled only by signaling from LagC. However, expression of lagC in a GBF-independent manner in a gbfA− null strain did not result in expression of the post-aggregation genes. Since GBF is necessary for accumulation of LagC and both the DNA binding protein and the LagC signal transduction pathway are necessary for expression of post-aggregation genes, GBF and LagC form a feed-forward loop. Such network architecture is a common motif in diverse organisms and can act as a filter for noisy inputs. Breaking the feed-forward loop by expressing lagC in a GBF-independent manner in a gbfA+ strain does not significantly affect the patterns of gene expression for cells developed in suspension with added cAMP, but results in a significant delay at the mound stage and asynchronous development on solid supports. This feed-forward loop can integrate temporal information with morphological signals to ensure that post-aggregation genes are only expressed after cell contacts have been made.
Databáze: OpenAIRE
Externí odkaz: