cDNA and deduced polypeptide sequence of a mouse selenoprotein P
| Autor: | Leopold Flohé, Peter Steinert, Gerhard Gross, Marion Ahrens |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Untranslated region
DNA Complementary Base pair Molecular Sequence Data Clinical Biochemistry SEP15 Biology Biochemistry Mice chemistry.chemical_compound Selenoprotein P Complementary DNA Animals Humans Amino Acid Sequence Selenoproteins Peptide sequence Gene Library Genetics Base Sequence integumentary system Selenocysteine cDNA library Proteins General Medicine Embryo Mammalian Molecular biology Rats chemistry Molecular Medicine Cattle Sequence Alignment Sequence Analysis |
| Zdroj: | BioFactors. 6:311-319 |
| ISSN: | 1872-8081 0951-6433 |
| DOI: | 10.1002/biof.5520060302 |
| Popis: | An 11-day embryonic Swiss Webster/NIH mouse cDNA library was screened with a partial murine selenoprotein P cDNA probe and a murine selenoprotein-P-type cDNA 1 clone of 2075 bp lenght was obtained. The clone contained a 5 0 -leader sequence of 132 bp length, the selenoprotein P coding frame, and 803 base pairs in the 3 0 untranslated region. Alignment and RNA folding studies revealed the presence of two well conserved selenocysteine inserting motifs in the 3 0 flanking region. The deduced polypeptide sequence comprises 380 residues including ten selenocysteines. Identical amino acid residues in homologous positions are 86%, 71%, and 64% when compared to the previously reported selenoprotein P sequences of rat, man, and cattle, respectively. The comparatively low similarity between the selenoprotein P sequences reported so far leaves open the question whether they belong to the same molecular clade. |
| Databáze: | OpenAIRE |
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