Comperative Evaluation of Vitek 2 and Etest Methods with the Referance Broth Microdilution for Antimicrobial Susceptibility Testing of Colistin Among Multi-Drug Resistant Gram-Negative Bacteria
| Autor: | Selin UĞRAKLI, Metin DOĞAN |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Gram-negative bacteria
biology Broth microdilution Antimicrobial susceptibility broth microdilution Infectious and parasitic diseases RC109-216 biochemical phenomena metabolism and nutrition equipment and supplies bacterial infections and mycoses biology.organism_classification Microbiology QR1-502 polycyclic compounds Colistin medicine bacteria Multi drug resistant antimicrobial resistance colistin Etest medicine.drug |
| Zdroj: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, Vol 26, Iss 3, Pp 518-527 (2021) |
| ISSN: | 1300-932X |
| Popis: | Introduction: Despite the increased need for colistin, especially in serious infections caused by carbapenem resistant gram-negative bacteria, problems and challenges regarding colistin susceptibility testing remain. The aim of this study was to evaluate the performance of Vitek 2, one of the commonly used automated systems, and Etest for colistin susceptibility testing compared with reference broth microdilution method (BMD). Materials and Methods: This study included 657 multi-drug resistance (MDR) Gram negative bacteria obtained from clinical samples; Negative control, Escherichia coli ATCC 25922 and Positive control, Escherichia coli NCTC 13846. The collected MDR isolates were performed colistin BMD according to ISO standard 20776-1, prospectively. Categorical agreement (CA), Very Major Error (VME), and Major Error (ME) rate were calculated. Acceptable performance was evaluated as; CA ≥90%; VME |
| Databáze: | OpenAIRE |
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