Single-Cell Imaging for Studies of Renal Uranium Transport and Intracellular Behavior
| Autor: | Akihiro Uehara, Haruko Yakumaru, Seiichiro Himeno, Hitomi Fujishiro, Kyoko Ayama, Izumi Tanaka, Hiroshi Ishihara, Shino Homma-Takeda, Masakazu Oikawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
inorganic chemicals
lcsh:QE351-399.2 Potassium Cell chemistry.chemical_element 010501 environmental sciences 01 natural sciences complex mixtures Nephrotoxicity uranium 03 medical and health sciences medicine distribution PIXE 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences lcsh:Mineralogy technology industry and agriculture imaging Geology Uranium Geotechnical Engineering and Engineering Geology S3 segments Cell nucleus medicine.anatomical_structure chemistry Cytoplasm Cell culture proximal tubules Biophysics Intracellular |
| Zdroj: | Minerals Volume 11 Issue 2 Minerals, Vol 11, Iss 191, p 191 (2021) |
| ISSN: | 2075-163X |
| DOI: | 10.3390/min11020191 |
| Popis: | Nephrotoxicity is the primary health effect of uranium exposure. However, the renal transport and intracellular behavior of uranium remains to be clearly elucidated. In the present study, the intracellular uranium distribution was examined with the cell lines derived from the S3 segment of mouse renal proximal tubules, which is a toxic target site of uranium, using microbeam-based elemental analysis. Uranium exposure at 100 μM for 24 h (non-toxic phase) was performed in S3 cells. Two types of measurement specimens, including those that are adhesive cell specimens and cryosection specimens, were examined for the positional relationship of the intracellular localization of uranium. Based on the combined results of single-cell imaging from the two types of cell specimens, uranium was distributed inside the cell and localized in the cytoplasm near the cell nucleus. In some cells, uranium was colocalized with phosphorus and potassium. The amount of uranium accumulated in S3 cells was estimated using thin section-standards. The mean uranium content of three adhesive cells was hundreds of femtogram per cell. Thus, we believe that single-cell imaging would be useful for studies on renal uranium transportation and cellular behavior. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|