2-Chloroethanol Induced Upregulation of Matrix Metalloproteinase-2 in Primary Cultured Rat Astrocytes Via MAPK Signal Pathways
| Autor: | Hongge Tang, Qi Sun, Fenghong Zhao, Yaping Jin, Yingjun Liao, Gaoyang Wang, Tong Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway p38 mitogen-activated protein kinases mitogen-activated protein kinase signal pathways Biology matrix metalloproteinase-2 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Western blot medicine Protein kinase A Original Research medicine.diagnostic_test Kinase General Neuroscience 2-chloroethanol astrocytes Molecular biology Cell biology 030104 developmental biology Phosphorylation 1 2-dichloroethane 030217 neurology & neurosurgery Immunostaining Neuroscience |
| Zdroj: | Frontiers in Neuroscience |
| ISSN: | 1662-453X |
| Popis: | This study was to explore the mechanisms underlying 1,2-dichloroethane (1,2-DCE) induced brain edema by focusing on alteration of matrix metalloproteinase-2 (MMP-2) in rat astrocytes induced by 2-chloroethanol (2-CE), an intermediate metabolite of 1,2-DCE in vivo. Protein and mRNA levels of MMP-2, and the phosphorylated protein levels of p38 MAPK (p-p38), extracellular signal regulated protein kinase (p-ERK1/2) and c-Jun N-terminal kinase (p-JNK1/2) in astrocytes were examined by immunostaining, western blot or real-time RT-PCR analysis. Findings from this study disclosed that protein levels of MMP-2 were upregulated by 2-CE in astrocytes. Meanwhile, protein levels of p-p38, p-ERK1/2 and p-JNK1/2 were also increased apparently in the cells treated with 2-CE. Moreover, pretreatment of astrocytes with SB202190 (inhibitor of p38 MAPK), U0126 (inhibitor of ERK1/2) or SP600125 (inhibitor of JNK1/2) could suppress the upregulated expression of p-p38, p-ERK1/2, and p-JNK1/2. In response to suppressed protein levels of p-p38 and p-JNK1/2, the protein levels of MMP-2 also decreased significantly, indicating that activation of MAPK signal pathways were involved in the mechanisms underlying 2-CE-induced upregulation of MMP-2 expression. |
| Databáze: | OpenAIRE |
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