Photodynamic Inactivation with Acridine Orange on a Multidrug‐resistant Mouse Osteosarcoma Cell Line
| Autor: | Shin Hashiguchi, Tsukasa Ashihara, Hiroaki Murata, Ginjirou Minami, Takako Nozaki, Hideyuki Takeshita, Katsuyuki Kusuzaki, Yasusuke Hirasawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
musculoskeletal diseases
Male Cancer Research Pathology medicine.medical_specialty medicine.medical_treatment Photodynamic therapy Bone Neoplasms Biology Multidrug resistance Article chemistry.chemical_compound Mice medicine Tumor Cells Cultured Neoplasm Animals Humans Chemotherapy Osteosarcoma Mice Inbred C3H Ploidies Acridine orange medicine.disease Molecular biology Drug Resistance Multiple Multiple drug resistance Oncology chemistry Photochemotherapy Cell culture Drug Resistance Neoplasm Acridine |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 1876-4673 0910-5050 |
| Popis: | Overcoming multidrug resistance (MDR) is an urgent issue to improve the prognosis of osteosarcoma patients. In this study, we undertook to clarify the effect of photodynamic therapy (PDT) with acridine orange (AO) on the MDR mouse osteosarcoma (MOS / ADR1) cell line, by comparing the outcome with the effect on a chemosensitive osteosarcoma (MOS) cell line. Cultured cells of MOS and MOS / ADR1 cell lines were exposed to AO at various concentrations for various times, followed by long- or short-term (10 or 1 min) illumination with blue light (466.5 nm) for excitation. Living cells were counted by means of the trypan blue exclusion test. The results showed that AO rapidly bound to DNA, RNA and lysosomes of living MOS and MOS / ADR1 cells and also that most tumor cells in both cell lines died rapidly (viability ratio to untreated cells: 1/1000) within 48 h under conditions of continuous or 15-min flash exposure to AO at concentrations above 1.0 microg/ml plus 10-min illumination with blue light. Even after flash exposure to AO at concentrations above 1.0 microg/ml plus 1-min illumination, the viability of MOS/ADR1 cells decreased to a viability ratio of less than 1/ 1000 within 72 h. Based on these results, we concluded that AO with photo-excitation has a strong cytocidal effect, not only on chemosensitive mouse osteosarcoma cells, but also on MDR mouse osteosarcoma cells. These results suggested that photodynamic therapy with AO may be a new approach to treating MDR human osteosarcomas. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text