Extremely short 20–33 nucleotide introns are the standard length inParamecium tetraurelia

Autor:	Dean Fraga, Robert D. Hinrichsen, Chris B. Russell
Rok vydání:	1994
Předmět:	Genetics Base Sequence biology Molecular Sequence Data Intron Nucleic acid sequence Group II intron DNA Protozoan biology.organism_classification Polymerase Chain Reaction Introns Complementary DNA Consensus Sequence RNA splicing Consensus sequence Animals Paramecium tetraurelia Paramecium
Zdroj:	Nucleic Acids Research. 22:1221-1225
ISSN:	1362-4962 0305-1048
DOI:	10.1093/nar/22.7.1221
Popis:	Paramecium tetraurelia has the shortest known introns as its standard intron length. Sequenced introns vary between 20 and 33 nucleotides in length. The intron sequences were discovered in genomic sequences coding for a variety of different proteins, including phosphatases, kinases, and low-molecular weight GTP-binding proteins. All intron sequences begin with the conserved dinucleotide GT and end with the conserved dinucleotide AG. The sequences are more AT rich than the Paramecium coding sequences. The identified sequences were confirmed as introns by sequencing several cDNA fragments. We report here analysis of the characteristics of 50 separate introns, including size, base composition, and a consensus sequence.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04e98daf71ca6b9da5c47fec08cd43e7 https://doi.org/10.1093/nar/22.7.1221 Zobrazit plný text záznamu