Encapsulated droplet interface bilayers as a platform for high-throughput membrane studies
| Autor: | D. K. Baxani, W. D. Jamieson, D. A. Barrow, O. K. Castell |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Soft Matter. 18:5089-5096 |
| ISSN: | 1744-6848 1744-683X |
| DOI: | 10.1039/d1sm01111a |
| Popis: | Whilst it is highly desirable to produce artificial lipid bilayer arrays allowing for systematic high-content screening of membrane conditions, it remains a challenge due to the combined requirements of scaled membrane production, simple measurement access, and independent control over individual bilayer experimental conditions. Here, droplet bilayers encapsulated within a hydrogel shell are output individually into multi-well plates for simple, arrayed quantitative measurements. The afforded experimental throughput is used to conduct a 2D concentration screen characterising the synergistic pore-forming peptides Magainin2 and PGLa. Maximal enhanced activity is revealed at equimolar peptide concentrations via a membrane dye leakage assay, a finding consistent with models proposed from NMR data. The versatility of the platform is demonstrated by performing in situ electrophysiology, revealing low conductance pore activity (∼15 to 20 pA with 4.5 pA sub-states). In conclusion, this array platform addresses the aforementioned challenges and provides new and flexible opportunities for high-throughput membrane studies. Furthermore, the ability to engineer droplet networks within each construct paves the way for “lab-in-a-capsule” approaches accommodating multiple assays per construct and allowing for communicative reaction pathways. |
| Databáze: | OpenAIRE |
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