Multiple interaction modes between saccharin and sweet taste receptors determine a species‐dependent response to saccharin
| Autor: | Xiangzhong Zhao, Meng Liu, Meng Cui, Bo Liu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
saccharin
QH301-705.5 digestive oral and skin physiology food and beverages allosteric binding site General Biochemistry Genetics and Molecular Biology Receptors G-Protein-Coupled Mice stomatognathic system Sweetening Agents Taste Animals sweet taste receptor Biology (General) inhibition interaction modes Research Articles psychological phenomena and processes Signal Transduction Research Article species‐dependent sweet taste |
| Zdroj: | FEBS Open Bio, Vol 12, Iss 2, Pp 494-499 (2022) FEBS Open Bio |
| ISSN: | 2211-5463 |
| DOI: | 10.1002/2211-5463.13355 |
| Popis: | Saccharin is a commonly used artificial sweetener that exhibits both sweetening and sweet inhibition activities. The species‐dependent response towards saccharin and the interaction between saccharin and the sweet taste receptor T1R2/T1R3 remain elusive. In this study we used mismatched chimeras of T1R2 and T1R3 and calcium mobilization functional analysis to reveal a detailed species‐dependent response towards saccharin of human, squirrel monkey, and mouse sweet taste receptors. Our findings, combined with previous results by others, suggest multiple and complex interaction modes between saccharin and the sweet taste receptor, which are helpful guidelines for effective modulation of the sweet taste by sweetener/sweet inhibitors. Saccharin exhibits both sweetening and sweet inhibition activities. Besides the orthosteric binding site in T1R2 for activating the human sweet taste receptor T1R2/T1R3, it appears that there are at least two allosteric inhibitory sites located in T1R2 and T1R3 respectively to inhibit the receptor activity, suggesting multiple and complex interaction modes between saccharin and the heterodimeric sweet taste receptor T1R2/T1R3. |
| Databáze: | OpenAIRE |
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