Establishment of an Enzyme-Linked Immunosorbent Assay for Detection of Hantavirus Antibody of Rats Using a Recombinant of Nucleocapsid Protein Expressed in Escherichia coli
| Autor: | Jiro Arikawa, Ikuo Takashima, Kazuo Goto, Akira Takakura, Toshio Itoh, Kumiko Yoshimatsu |
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| Rok vydání: | 2003 |
| Předmět: |
Orthohantavirus
Hantavirus Infections Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral medicine.disease_cause Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology law.invention Bacterial Proteins Antigen law Escherichia coli medicine Animals Serologic Tests Rats Wistar Fluorescent Antibody Technique Indirect Hantaan virus Hantavirus chemistry.chemical_classification General Veterinary Strain (chemistry) Antibody titer virus diseases General Medicine Nucleocapsid Proteins Virology Recombinant Proteins Rats Enzyme chemistry Recombinant DNA Female Animal Science and Zoology Biomarkers |
| Zdroj: | Experimental Animals. 52:25-30 |
| ISSN: | 1881-7122 1341-1357 |
| Popis: | A recombinant nucleocapsid protein of Hantaan virus (HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay (IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR-11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples. |
| Databáze: | OpenAIRE |
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