Thirst-associated preoptic neurons encode an aversive motivational drive
| Autor: | Lief E. Fenno, Laura A. DeNardo, Kyle M. Loh, Liqun Luo, Michael Z. Chen, Karl Deisseroth, Charu Ramakrishnan, Cindy D. Liu, William E. Allen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell type Drinking Drinking Behavior Stimulation Optogenetics Biology Article Cell Line Thirst Mice 03 medical and health sciences Neural activity medicine Humans Animals Median preoptic nucleus Drive Neurons Motivation Multidisciplinary Dehydration Gene Expression Profiling TNF Receptor-Associated Factor 2 Preoptic Area Preoptic area 030104 developmental biology Excitatory postsynaptic potential Single-Cell Analysis medicine.symptom Neuroscience |
| Zdroj: | Science. 357:1149-1155 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.aan6747 |
| Popis: | Water deprivation produces a drive to seek and consume water. How neural activity creates this motivation remains poorly understood. We used activity-dependent genetic labeling to characterize neurons activated by water deprivation in the hypothalamic median preoptic nucleus (MnPO). Single-cell transcriptional profiling revealed that dehydration-activated MnPO neurons consist of a single excitatory cell type. After optogenetic activation of these neurons, mice drank water and performed an operant lever-pressing task for water reward with rates that scaled with stimulation frequency. This stimulation was aversive, and instrumentally pausing stimulation could reinforce lever-pressing. Activity of these neurons gradually decreased over the course of an operant session. Thus, the activity of dehydration-activated MnPO neurons establishes a scalable, persistent, and aversive internal state that dynamically controls thirst-motivated behavior. |
| Databáze: | OpenAIRE |
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