Evaluation of the Biodegradability of Polyurethane and Its Derivatives by Using Lipase-Displaying Arming Yeast
| Autor: | Seiji Shibasaki, Akihiko Kondo, Mamoru Tanaka, Takanori Tanino, Ai Kawabata, Mitsuyoshi Ueda |
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| Rok vydání: | 2009 |
| Předmět: |
biology
Polyurethanes Public Health Environmental and Occupational Health Lipase Saccharomyces cerevisiae Arming yeast Biodegradation biology.organism_classification Applied Microbiology and Biotechnology Yeast Fungal Proteins Polyester chemistry.chemical_compound Biodegradation Environmental chemistry Galactitol biology.protein Organic chemistry Candida antarctica Ethylene glycol Polyurethane |
| Zdroj: | Biocontrol Science. 14:171-175 |
| ISSN: | 1884-0205 1342-4815 |
| DOI: | 10.4265/bio.14.171 |
| Popis: | Candida antarctica lipase B (CALB) has been used to polymerize and degrade polyesters. We developed a convenient method for investigating the biodegradability of plastics that involves the use of CALB-displaying "arming yeast." Polyurethane containing dulcitol units was prepared and used as the model material. Additionally, standard polyurethane with no dulcitol units was prepared by reacting 2,4-toluene diisocyanate with ethylene glycol. These polymers were incubated with CALB-displaying yeast cells. The polyurethane containing dulcitol was degraded, while the standard polyurethane was relatively unaffected. Arming yeast displaying appropriate enzymes can be used to investigate the biodegradability of synthetic plastics. It was also revealed that arming yeasts were applicable to evaluate the degradation of the film state of polyurethane. |
| Databáze: | OpenAIRE |
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