Dimethyl sulfoxide (DMSO) attenuates the inflammatory response in the in vitro intestinal Caco-2 cell model
| Autor: | Olivier Toussaint, Yves-Jacques Schneider, Yvan Larondelle, Thomas Raas, Alexandrine During, Sylvie Hollebeeck, Neil Piront |
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| Rok vydání: | 2011 |
| Předmět: |
Interleukin-1beta
Messenger Anti-Inflammatory Agents Toxicology Dinoprostone Dose-Response Relationship chemistry.chemical_compound Humans Dimethyl Sulfoxide RNA Messenger Chemokine CCL2 Dose-Response Relationship Drug Interleukin-6 Dimethyl sulfoxide Cell growth NF-kappa B Interleukin Chemotaxis General Medicine Molecular biology In vitro Dose–response relationship chemistry Biochemistry Caco-2 RNA Arachidonic acid Caco-2 Cells Drug |
| Zdroj: | Toxicology Letters. 206:268-275 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/j.toxlet.2011.08.010 |
| Popis: | This study aimed to investigate dose effects of dimethyl sulfoxide (DMSO) (0.05-1%) on the intestinal inflammatory response in confluent- and differentiated-Caco-2 cells stimulated with interleukin (IL)-1β or a pro-inflammatory cocktail for 24 h. Cyclooxygenase-2 (COX-2) activity was assayed by incubating inflamed cells with arachidonic acid and then measuring prostaglandin-E(2) (PGE(2)) produced. Soluble mediators (IL-8, IL-6, macrophage chemoattractant protein-1 (MCP-1), and COX-2-derived PGE(2)) were quantified by enzyme immunoassays and mRNA expression of 33 proteins by high throughput TaqMan Low Density Array. Data showed that DMSO decreased induced IL-6 and MCP-1 secretions in a dose-dependent manner (P |
| Databáze: | OpenAIRE |
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